Abstract: Objective To observe changes in cytokine secretion induced by Zika virus （ZIKV） infection in human neuroblastoma SH-SY5Y and explore the mechanism of ZIKV affecting nervous system dysfunction. Methods A model of SH-SY5Y cells infected with ZIKV was established and the cell viability was detected by MTT assay. Cell culture supernatants were harvested at 24, 48, and 72 hours. Concentrations of IL-1β, IL-2, IL-4, IL-5, IL-13, IL-18, IFN-γ, TNF-α, IL-12P70, and GM-CSF were measured simultaneously and quantitatively using the Bio-Plex^? 200 system. Results The cell survival rates at 24, 48, and 72 hours in the virus infection group were lower than those in the normal control group （all for P <0.01）. The levels of IL-1β, IL-5, IFN-γ, TNF-α, IL-12P70, and GM-CSF secreted by the virus infection group （24-72 h） were higher than those of the control group （all for P <0.01）. The levels of IL-1β, IL-12P70, and GM-CSF secreted by the virus infection group （48 h） were higher than those of the control group （48 h）, and the level of TNF-α secreted by the virus infection group （72 h） was higher than that of the control group （72 h） （P <0.05 or P <0.01）. Conclusion SH-SY5Y cells infected with ZIKV resulted in the decrease in cell viability and the secretion of relatively high concentration of pro-inflammatory cytokines in a time-dependent manner.

Key words: Zika virus, Human neuroblastoma cell, Cytokines