Abstract: Objective To understand the characteristics, inherent law and false-positive rate of HIV-antibody detection in pregnant women so as to provide reference for intervention strategies of mother-to-child transmission of HIV. Methods Retrospective analysis of re-inspection and confirmation was conducted for pregnant women who were HIV-antibody preliminary screening positive in Guangzhou City during 2004 to 2013. Results Amongst 808 samples for re-inspection, the coincidence rate of preliminary screening and re-inspection was 66.58%（538/808）, the coincidence rate of confirmation test was 59.48%（320/538), and the uncertain rate was 11.52%（62/538）. Of 62 uncertain samples, 45 were followed up and 3 of them were HIV-seroconversion with a conversion rate of 6.67%（3/45）. The samples with S/CO<4 were all confirmed negative. When the S/CO value was equal to or higher than 4, the samples turned to be positive. The positive rate increased with the increase of S/CO value. Conclusion The false positive rate was high in HIV antibody detection in pregnant women and the diagnoses of HIV infection should be considered with experiment results and epidemiological history information in maternal intervention.