Abstract: Objective To explore the subtype distribution characteristics and drug resistance of human immunodeficiency virus type 1 （HIV-1） among HIV-1-infected individuals in Dongguan. Methods Plasma samples of 50 HIV infected individuals without antiviral treatment were collected from October 2015 to February 2016 in Dongguan Municipal Center for Disease Control and Prevention. The viral RNA of HIV-1 was extracted, the pol gene was amplified by nest RT-PCR and the PCR products were sequencd. The obtained nucleic acid sequences were submitted to the Stanford University HIV Drug Resistance Database（http：//hivdb. stanford. edu）for drug resistance analysis. Results Among the 50 plasma samples, the pol gene sequences of 35 samples were successfully amplified. Of the 35 subjects, 88. 6% （31/35） were male and 65. 7% （23/35） were infected through the sexual transmission. Among the 35 pol gene sequences, 37. 1% （13/35） were CRF01_AE, 37. 1% （13/35） were CRF07_BC, 14. 3% （5/35） were subtype B, and 11. 4% （4/35） were CRF55_01B. Mutations related to drug resistance were found in 11 （31. 4%） of 35 pol gene sequences. Major mutation subtypes of HIV-1 were CRF01_AE and CRF55_01B. The rate of primary drug resistance was 11. 4%（4/35）. Conclusion The genetic subtypes of HIV-1 were diverse and the main recombinant subtypes were CRF01_AE and CRF07_BC in Dongguan City. More attention should be paid to the primary drug resistance mutations in gene sequence.

Key words: Human immunodeficiency virus type 1 （HIV-1）, Genetic subtype, Drug resistance