Abstract: Objective To understand HIV/AIDS epidemic and testing status in Guangdong Province so as to provide scientific evidence for policy and strategy making. Methods Data were collected from the HIV/AIDS reporting system, sentinel surveillance system, and specific surveys from 2000 to 2013 in Guangdong Province and analyze by using descriptive epidemiology method. Results A total of 49 038 HIV/AIDS cases were reported from 2000 to 2013 in Guangdong Province. The annual reported HIV/AIDS cases increased from 326 in 2000 to 7 631 in 2013, with an average annual increase rate of 23.2%. Amongst all the cases, 78.6% were males and 21.4%, females. Their age distributions were mainly in 30 to 39 years and 20 to 29 years, accounting for 37.9% and 29.1% respectively. Occupations were mainly the unemployed (23.1%) and peasants (19.2%). Of them, 60.9% had Guangdong provincial residency. HIV transmission route was mainly the heterosexual contact (46.7% of all cases), followed by injection drug use (34.9%). HIV antibody positive rates were high among spouses or sexual partners of HIV positive people (11.735%, 280/2 386), children of HIV positive mothers (9.381%, 50/533), people from voluntary HIV counseling and testing clinics (1.569%, 3 920/249 775), and people from detoxification centers (1.299%, 2 486/191 399). Conclusion The HIV epidemic was increasing in Guangdong Province from 2000 to 2013. The transmission mode of HIV has changed from injection drug use to sexual contact. Corresponding policy and strategies were urgently needed to expand the HIV testing among high risk population of sexual transmission in Guangdong Province.