Abstract: Objective To explore a suitable method for detection of hepatitis E virus (HEV) in pig liver sample by TaqMan real time RT-PCR method. Methods Two methods, homogenized direct extraction method (Method A) and polyethylene glycol precipitation method (Method B), were used for concentration of HEV from swine liver samples. Based on the specific primers and probes of the four genotypes of HEV, a TaqMan real-time RT-PCR method was developed and its accuracy and sensitivity were evaluated. Real time RT-PCR was used to detect HEV from swine livers with induced infection of HEV. Performances of the two methods were compared. Results The established TaqMan real-time RT-PCR standard curve showed a good linear relationship in range of 102 - 108 copies/μL. The correlation coefficient (R2) of the standard curves with plasmid DNA was 0.999. The coefficients of variation values of the different diluted plasmid DNA were detected between 0.11% and 2.47% and between 6.62% and 2.69% in the same or different repeated experimental groups. The efficiency of Method A and Method B was similar with a detection limit of 102 copies/μL. Conclusion The TaqMan real-time RT-PCR assay was established with high sensitivity, good stability and strong specificity, suitable for quantitative detection of HEV. The homogenized direct extraction method is more rapid for HEV concentration from swine livers and suitable for long-term surveillance of HEV.