Abstract: ObjectiveAn ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established for determination of α-solanine and α-chaconinee in food poisoning samples including potatoes, processed food, vomit, serum, and urine. MethodsThe samples were extracted with 5% acetic acid by ultrasonic instrument. For potato sample, the supernatant was diluted with acetonitrile and detected by UPLC-MS/MS. For samples of processed food, vomit, serum, and urine, the supernatants were cleaned-up by solid-phase extraction with a mixed mode cation exchange, and eluted with acetonitrile (containing 5% ammonia water). Then the purified solution was concentrated by nitrogen, dissolved with acetonitrile-water (90+10, v/v) and cleaned by 0.22 μm millipore filter. The sample extract was separated on a Waters UPLC BEH Amide column by gradient elution in 10 minutes with acetronitrile-water (containing 2 mmol/L ammonium acetate) as mobile phase. The filtrate was detected by UPLC-MS/MS, identified by electrospray ionization in positive mode using multiple reaction monitoring, and quantified with external standards. ResultsThe calibration curves of α-solanine and α-chaconinee in several poisoning samples showed good linearity in the range of 1.0-200.0 μg/L with correlation coefficient in the range of 0.998 8-0.999 7. The detection limit of the method were 0.60 μg/kg (α-solanine) and 0.21 μg/kg (α-chaconinee) for potato, 0.08 μg/L (α-solanine) and 0.04 μg/L(α-chaconinee) for serum and urine. The recoveries of three spiking levels ranged from 82.6% to 105.5%, andRSDs of 1.93% -5.11% were obtained. ConclusionThis method is simple, rapid, and accurate for the determination of residues of α-solanine and α-chaconinee in poisoning samples and can be used for emergency detection of solanine poisoning.