Abstract: Objective To study the genotoxicity and teratogenicity of Wanglaoji herbal tea. Methods NIH mice were gavaged with Wanglaoji herbal tea extract at doses of 2.5, 5.0, and 10.0 g/kg?bw（equivalent to 16.65, 33.30, and 66.60 g/kg?bw of herbal crude drugs）to examine sperm abnormality and micronuclei of bone marrow using bone marrow cell micronucleus test and mice sperm abnormality test. Salmonella typhimurium strains of TA97, TA98, TA100 and TA102 were used in Ames tests at the doses of 5 000, 1 000, 200, 40, and 8 μg/plate of Wanglaoji herbal tea extract（equivalent to 33.30, 6.66, 1.33, 0.27, and 0.05 mg/plate of herbal crude drugs），to determine each state's number of revertant colonies. Pregnant SD female rats were exposed to doses of 1.63, 3.25, 6.50 g/kg?bw of Wanglaoji herbal tea extract（equivalent to 10.86, 21.65, 43.29 g/kg?bw of herbal crude drugs） through stomach feeding for conducting the teratogenicity test. Embryonic and fetal developmental parameters were surveyed. External, skeletal and internal organ anomalies were examined. Both negative and positive control groups were set in bone marrow cell micronucleus test, mouse sperm abnormality test and Ames test. Only negative control group was set in teratogenicity test. Results No significant differences were found in micronucleus rates of negative control group and three dose groups of Wanglaoji herbal tea extract (1.8‰, 2.0‰, 2.4‰, and 2.2‰ for male mouse group and 2.0‰, 2.4‰, 2.0‰, and 2.6‰ for female mouse group, respectively) and the sperm deformity rates (17.2‰, 14.8‰, 18.0‰, and 19.0‰, respectively) (all P>0.05）. Compared with the control group, Ames test (in presence or absence of S9) did not show Wanglaoji herbal tea extract groups a 2 fold increase in the spontaneous reversion colonies. The result of Ames test was negative. The result of teratogenicity test showed that no significant difference was found in activity, weight gain, live birth rate, stillbirth rate, and absorbed fetus rate of pregnant mice between the control and Wanglaoji herbal tea extract groups (all P>0.05）. There was no difference in the rates of fetal mortality and embryos absorption, as well as in the increase of weight, body length and tail length of fetal mice between the control and Wanglaoji herbal tea extract groups（all P>0.05）. No external or internal organ or skeletal anomalies was observed. The result of teratogenicity test was negative. Conclusion Under the experimental conditions and dose ranges in this paper, the genotoxicity and teratogenic effects of Wanglaoji herbal tea extract were not observed.