South China Journal of Preventive Medicine ›› 2020, Vol. 46 ›› Issue (4): 355-358.doi: 10.12183/j.scjpm.2020.0355

• Original Article • Previous Articles     Next Articles

Modulatory effect of matrix Gla protein on bone mass and underlying mechanism

LI Jing, ZHAO Li-ting, ZHANG Yan   

  1. Center for Translational Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi’an 710061, China
  • Received:2020-04-05 Online:2020-08-20 Published:2020-09-16

Abstract: Objective To detect effects of matrix Gla protein (MGP) on bone mass and the underlying mechanism. Methods MGP expression was interfered with recombinant adeno-associated virus (rAAV) in mice. The bone mass was examined by microCT analysis. The formation of osteoclasts was determined by tartrate resistant acid phosphatase (TRAP) staining. The serum level of TRAP5b was assessed by ELISA. Results Compared with the control group, the bone volume fraction of mice treated with MGP interference was significantly reduced, the number and the thickness of the trabecular bone were also significantly reduced, and the interval of the trabecular bone was significantly increased (P<0.01 for all). The number of TRAP-positive osteoclasts in mouse femoral slices increased, and the level of serum TRAP5b increased significantly (P<0.01). Conclusion MGP may regulate bone mass by inhibiting osteoclast formation and bone resorption.

Key words: MGP, Osteoporosis, Osteoclasts

CLC Number: 

  • R336