Abstract: Objective To know the distribution and drug resistance trend of pathogens in intensive care unit （ICU） patients of a hospital in Guangzhou from 2018 to 2020, and provide a strong basis for formulating reasonable infection control measures. Methods The results of pathogens and drug susceptibility detected from ICU patients in a hospital in Guangzhou from 2018 to 2020 were collected, and the data were statistically analyzed using software WHONET 5.6 and SPSS 25. Results From 2018 to 2020, a totally 1 978 strains of non-repeating pathogens were isolated from 7 164 samples, mainly Gram-negative bacteria, 338 （61.0%）, 439 （57.2%）, and 387 （59.0%） strains each year respectively. Acinetobacter baumannii maintained a high resistance rate to most antibiotics （>33.3%）, Escherichia coli maintained a good sensitivity to carbapenems （≤4.8%）, while Pseudomonas aeruginosa maintained the opposite （>27.6%）. The drug resistance of Methicillin-resistant staphylococcus aureus and carbapenem-resistant Enterobacteriaceae had an upward trend （both P<0.01）. Conclusion To effectively prevent the emergence and cross-transmission of multi-drug resistant organisms, we should strengthen the monitoring of the distribution of pathogens and the trend of drug resistance in ICU patients, strictly implement the control measures such as hand hygiene, environmental cleaning and instrument disinfection, and strengthen the supervision of the rational use of key antibacterial drugs.

Key words: Intensive care unit, Drug resistance, Multi-drug resistant organisms