Objective To explore the mental status and its influencing factors of pregnant women in Haikou City, so as to provide a scientific basis for improving maternal and infant health. Methods From January 2020 to September 2022, pregnant women who underwent obstetric examinations and established records at 4 medical institutions in Haikou City were selected to investigate by a self-designed general situation questionnaire and Symptom Check List-90 （SCL-90）. The mental status of pregnant women in Haikou City was analyzed by descriptive epidemiological method, and the related influencing factors were analyzed by univariate and multivariate Logistic regression analysis methods. Results The average score of SCL-90 of 4 522 pregnant women was （139.75±21.67）, the total score of Pregnancy Pressure Scale （PPS） was （65.51±6.18）, the total score of General Well Being Schedule （GWBS） was （69.65±3.63）, and the total score of childbirth attitudes questionnaire （CAQ） was （38.42±4.24）. Pearson correlation analysis showed that the score of SCL-90 was positively correlated with the score of PPS and CAQ （P< 0.01）, and negatively correlated with the score of GWBS （P<0.01）. Among 4 522 pregnant women, there were 801 pregnant women with a total score of over 160 on the SCL-90. Multivariate Logistic regression analysis showed that age ≥ 35 years （OR=5.807）, primipara （OR=2.492）, lower education level （OR=4.740）, diabetes （OR=1.589）, hypertension （OR=1.560）, preeclampsia （OR=1.940）, premature rupture of membranes （OR=1.754）, and sleep quality score≥ 7 （OR=4.225） were independent risk factors for positive SCL-90 scores of pregnant women. Conclusions There are some pregnant women in Haikou City with poor mental status. Psychological intervention should be carried out according to the independent risk factors of pregnant women's mental status to improve the level of mental health of pregnant women.

Objective To investigate the correlation of body composition and dietary habits to the sleep quality of students in a university of traditional Chinese medicine in Beijing, and to provide evidence for improving sleep quality in this group. Methods From October to November 2021, a stratified random sampling method was adopted to randomly select students from a medical university in Beijing. An electronic questionnaire was used to collect their general information, dietary habits, and sleep quality, and body composition was measured on-site. Spearman correlation was used to analyze the relationship between sleep quality, dietary habits, and body composition. Results Among 235 medical students surveyed, 55 （23.40%） had poor sleep quality, among which 42 （76.36%） were female students, and thin students accounted for the most （52.73%）. The sleep quality of students of different majors, genders, and body types was statistically significant （all P<0.01）. Sleep frequency, daytime dysfunction 1, and daytime dysfunction 2 were related to body type （P<0.05 or P<0.01）, and the obese type had the highest score. Sleep quality score was negatively correlated with vegetables, fruits, beans, and breakfast （P<0.05 or P<0.01）, and positively correlated with livestock and poultry meat, fried food, night snack, coffee, carbonated beverage, fruit juice, alcoholic beverage, chocolate （P<0.05 or P<0.01）. Conclusions Sleep quality is related to body composition and dietary habits. By correcting bad dietary habits to reduce body fat percentage and improve body shape, sleep quality may be improved.

Objective To analyze the traffic injury disease burden of registered residents in Guangdong Province from 2005 to 2015, and to provide scientific basis for the development of traffic injury prevention and control strategies. Methods The traffic injury mortality, standardized mortality, years of life lost （YLL） due to early death, years lost due to disability （YLD）, disability adjusted life year （DALY）, life expectancy, and life expectancy regardless of death cause were calculated using the monitoring data of traffic injury causes of death of registered residents in Guangdong Province from 2005 to 2015. Percentage of annual change （APC） was used to describe the change trend of the above indexes. Results The mortality rate of traffic injury among registered residents in Guangdong Province decreased from 20.20/100 000 in 2005 to 11.89/100 000 in 2015 （APC=-5.45%,P<0.01）. The mortality rate of male traffic injury was higher than that of female （all P<0.05）. From 2005 to 2015, the YLL rate and DALY rate of traffic injury showed a decreasing trend （APC=-6.95%,-5.92%,both P<0.01）, while the YLD rate showed an increasing trend （APC=2.43%,P<0.01）. In 2005, the DALY rate of traffic injury among people over 20 years old decreased with the increase of age; In 2015, the DALY rate of traffic injury increased with the increase of age, and the DALY rate of traffic injury among people aged 5-60 years old decreased compared with that in 2005, and the biggest decrease （68%） was in the group of 25-29 years old, while the burden of the group of 50-70 years old was higher, maintain at a high level. The life added value after the elimination of traffic injury decreased from 0.52 years in 2005 to 0.32 years in 2015 （P>0.05）. Conclusions Traffic injury has become the main accidental injury cause of death of registered residents in Guangdong Province. Males and people over 50 years old should be taken as the key groups for traffic injury intervention.

Objective To understand the current situation of prevention and treatment of drinking water-type endemic fluorosis in Xinjiang Production and Construction Corps （hereinafter referred to as the Corps）, and to assess the non-carcinogenic health risk of fluoride in drinking water. Methods In 2019, all drinking water fluorosis areas in the Corps were monitored, including 11 divisions and 62 regiments. The monitoring contents included the operation of the water improvement project, the fluoride content in the water, and the prevalence of dental fluorosis among children aged 8 to 12 years. The risk assessment model of the United States Environmental Protection Agency （US EPA） was used to assess the health risk of fluoride intake through drinking water. Results In the regimental towns of drinking water-type fluorosis area, the water improvement rate was 100.00% （62/62）, the water improvement project was 100.00% in normal operation, the water fluoride content was 0.09 - 1.94 mg/L, and the qualified rate was 96.77% （60/62）; The water improvement rate of the farming and animal husbandry company was 100.00% （611/611）, the water fluoride content was 0.01-7.27 mg/L, and the qualified rate was 90.02% （550/611）; The total qualified rate was 90.64% （610/673）. The prevalence rate of dental fluorosis in children was 3.65% （1 564/42 839）, the dental fluorosis index was 0.07, and there was no epidemic of dental fluorosis. There was a statistically significant difference among divisions （P<0.01）. The prevalence rate of dental fluorosis in the 14th division was the highest （11.48%）; The non-carcinogenic health risk hazard coefficients of fluoride in both males and females aged 6 to 9 years were the highest, which were 0.29 and 0.30 respectively. Conclusions The water improvement rate of the drinking water-type fluorosis area of the Corps is high, the condition of children's dental fluorosis has been controlled. However, the water fluoride in some regimental towns and agricultural and animal husbandry companies still exceeds the standard, so it is necessary to strengthen the supervision of water improvement projects and the monitoring of water fluoride content.

Objective To investigate the prevalence of thyroid nodules and iodine nutrition of school-age children in Wuhan, and to explore the correlation between them, to provide a basis for formulating prevention and treatment strategies for thyroid nodules and iodine deficiency disorder in children. Methods From 2019 to 2021, according to the east, west, south, north, and middle positions of the eight districts in Wuhan, one primary school was selected from each positions, 40 non-boarding students aged 8-10 years from each primary school were selected for thyroid B-ultrasound examination, their height and weight were measured, and urine samples and household salt samples were randomly tested once. The Chi-square test, rank sum test, and binary Logistic regression model were used to analyze the influencing factors of thyroid nodules and their correlation with urinary iodine levels. Results A total of 1 600 school-age children aged 8-10 years were investigated. The median urinary iodine was 245.51 μg/L, and the median urinary iodine exceeded the appropriate level in children of different genders, ages, and regions. The median iodine content of edible salt was 23.70 mg/kg in children aged 8-10 years, and the qualified iodized salt consumption rate was 95.63% （1 530/1 600）. The median urinary iodine content was positively correlated with the median iodine content of edible salt （P<0.01）. The detection rate of thyroid nodules in school-age children aged 8-10 years was 4.94% （79/1 600）. Girls （OR=1.71） and high urinary iodine levels （OR=1.39） were risk factors for thyroid nodules in children. The detection rate of thyroid nodules was positively correlated with the urinary iodine level of children （P<0.01）. Conclusions The prevalence of thyroid nodules in school-age children aged 8-10 years in Wuhan is lower than that in most areas of China. The iodine nutrition level is above the appropriate level. High urinary iodine levels can increase the risk of thyroid nodules in children.

Objective To explore the epidemic of dengue fever and analyze the genotype of dengue virus serotgpe Ⅰ （DENV-1） and characteristic of E gene in Dongguan from 2014 to 2019. Methods A total of 962 serum samples of clinically diagnosed suspected dengue fever cases were collected from various town and street hospitals in Dongguan from 2014 to 2019. The level of IgM antibody of 729 serum samples were detected by enzyme-linked immunosorbent assay （ELISA）. All 962 serum samples were detected by real time PCR method. DENV-1 samples in nucleic acid positive samples were isolated and identified by cell culture, and their E gene sequences were sequenced and phylogenetic tree analysis was carried out. Results From 2014 to 2019, dengue fever in Dongguan was prevalent from July to November and peaked from September to October. Two hundred and sixty-one samples of IgM antibody positive were detected, and the positive rate was 35.90%. Three hundred and sixty-two samples were positive for DENV nucleic acid. Among the positive samples, 311 were DENV-1 positive, accounting for 85.9%, and 35 strains of DENV-1 were isolated. Sequence alignment and evolutionary of Egene analysis showed that the nucleotide homology among the 35 strains of DENV-1 was 89.8%-100.0%. They were genotype Ⅰ （29 strains）, genotype Ⅱ （1 strain） and genotype Ⅴ （5 strains）. The 35 strains had higher homology with strains from Guangzhou and Zhejiang and the southeast Asian countries such as Viet Nam and Philippines and India. Conclusions The dominant type of dengue virus circulating in Dongguan during 2014-2019 is genotype Ⅰ DENV-1. The epidemic mode of dengue fever may be local outbreaks caused by imported cases mainly from the cities in or around Guangzhou and southeast Asian countries. We must be wary of the imported genotype Ⅴ DENV-1 that causes dengue fever outbreak.

Objective To explore the reliability and validity of the Chinese version of the Quality of Life Scale for Adult Cancer Survivors （QLACS） in patients with cervical cancer. Methods Cervical cancer patients treated at a tertiary hospital in Jiangsu Province from May 2021 to August 2022 were selected for the study using a convenient sampling method. The Chinese version of the QLACS scale was used for the questionnaire survey. Cronbach's α and half-fold coefficient were used to evaluate the internal consistency of the scale. The content validity of the scale was evaluated by the correlation coefficient between the score of each item and the total score and the intra-group correlation coefficient, which was tested using the expert evaluation method. The structural validity of the scale was evaluated by exploratory factor analysis. Results The Chinese version of the QLACS scale contains 47 items and 12 dimensions, which are divided into general aspects and cancer-specific aspects. The results of the exploratory factor analysis showed that 12 common factors were extracted from the 47 evaluated items. The KMO value of the Chinese version of the QLACS scale was 0.854, and the Bartlett test of sphericity reached the significance test level （P <0.01）. In the content validity index, I-CVI was 0.84-1.00, S-CVI/UA was 0.84, and S-CVI/Ave was 0.94. The Cronbach's α of the Chinese version of the QLACS scale was 0.924, and the half-fold coefficient was 0.904. The Cronbach's α of the 12 dimensions ranged from 0.801 to 0.924, and the half-fold coefficient ranged from 0.826 to 0.934. Conclusion The Chinese version of the QLACS scale has shown good reliability and validity in cervical cancer patients and can be used to evaluate the quality of life of cervical cancer survivors.

Objective To know the distribution and drug resistance trend of pathogens in intensive care unit （ICU） patients of a hospital in Guangzhou from 2018 to 2020, and provide a strong basis for formulating reasonable infection control measures. Methods The results of pathogens and drug susceptibility detected from ICU patients in a hospital in Guangzhou from 2018 to 2020 were collected, and the data were statistically analyzed using software WHONET 5.6 and SPSS 25. Results From 2018 to 2020, a totally 1 978 strains of non-repeating pathogens were isolated from 7 164 samples, mainly Gram-negative bacteria, 338 （61.0%）, 439 （57.2%）, and 387 （59.0%） strains each year respectively. Acinetobacter baumannii maintained a high resistance rate to most antibiotics （>33.3%）, Escherichia coli maintained a good sensitivity to carbapenems （≤4.8%）, while Pseudomonas aeruginosa maintained the opposite （>27.6%）. The drug resistance of Methicillin-resistant staphylococcus aureus and carbapenem-resistant Enterobacteriaceae had an upward trend （both P<0.01）. Conclusion To effectively prevent the emergence and cross-transmission of multi-drug resistant organisms, we should strengthen the monitoring of the distribution of pathogens and the trend of drug resistance in ICU patients, strictly implement the control measures such as hand hygiene, environmental cleaning and instrument disinfection, and strengthen the supervision of the rational use of key antibacterial drugs.

Objective To analyze the distribution of allergens and the influencing factors in patients with allergic rhinitis. Methods Employees of a science and technology park who underwent physical examinations at Beijing Aerospace General Hospital from January to December 2022 were selected for the study. Allergic rhinitis was diagnosed by questionnaire, physical examination, and allergen-specific IgE antibody testing. Descriptive methods were used to analyze the prevalence of allergic rhinitis and distribution characteristics of allergens. The influencing factors of allergic rhinitis were analyzed by univariate and multivariate analysis methods. Results A total of 11 541 employees were surveyed, among whom 1 849 were diagnosed with allergic rhinitis, with a prevalence rate of 16.02%. After allergen testing, there were 1 610 patients with inhaled allergens, for a positive rate of 87.07%; 484 patients with food allergens, for a positive rate of 26.18%. The top five inhaled allergens were dust mites （52.68%）, house dust （40.51%）, tree pollen （27.69%）, mugwort （13.90%）, and ragweed （11.52%）. The top five food allergens were cod/lobster/scallop （6.65%）, soybean （5.84%）, freshwater fish （4.98%）, crab （3.52%）, and shrimp （3.14%）. Among them, 736 patients were single allergens, accounting for 39.26%, and 1 113 patients had two allergens and over, accounting for 60.74%. Multivariate Logistic regression analysis showed that aged 18-29 years （OR=2.159）, aged 30-39 years （OR = 2.115）, BMI≥28.0 kg/m² （OR=1.717）, management position （OR= 1.358）, previous allergic history （OR=1.765）, parental allergic rhinitis （OR=1.444）, smoking or passive smoking （OR=1.513） were the influencing factors for allergic rhinitis. Conclusions The prevalence of allergic rhinitis among the occupational population in Beijing is high, and the main allergens are inhaled allergens. Age, BMI≥28 kg/m², employee in management position, previous allergic history, parental allergic rhinitis, and smoking or passive smoking were the influencing factors for allergic rhinitis.

Objective To analyze the characteristics of Mycoplasma pneumoniae infection in children and investigate the risk factors of infection, so as to provide the reference for the prevention and control of Mycoplasma pneumoniae infection in children. Methods Children diagnosed with pneumonia and treated at Changzhi Lucheng District People's Hospital from January 2018 to December 2022 were included in the study. Data on gender, age, onset season, clinical symptoms, chest auscultation, pulmonary imaging manifestations （such as patchy or large flake dense shadow）, white blood cell count, history of mycoplasma infection, and other relevant factors were collected. The rate of Mycoplasma pneumoniae infection in children was calculated based on the results of mycoplasma antibody detection in venous blood, and the distribution characteristics of infected cases were analyzed. Univariate and multivariate Logistic regression analyses were performed to identify risk factors for Mycoplasma pneumoniae infection in children. Results A total of 3 891 valid cases were collected, with a validity rate of 95.23%. Mycoplasma pneumoniae infections were detected in 718 cases, with an infection rate of 18.45% （718/3 891）. There were more boys （56.27%） than girls （43.73%） infected with Mycoplasma pneumoniae, and the highest proportion （41.50%） of infections occurred amongst children aged 4-6 years, followed by children aged 1-3 years, 7-12 years, and 3 months to 1 year old. Furthermore, infections were most common during winter （39.83%）, followed by autumn, spring, and summer. The most prevalent clinical symptom was cough （89.00%）, followed by fever, and shortness of breath. Multivariate Logistic regression analysis indicated that older age （OR=1.963）, cough （OR=2.768）, patchy or large flake dense shadows on pulmonary imaging （OR=2.983）, elevated white blood cell count （OR=2.161）, and history of mycoplasma infection （OR=1.436） were risk factors for Mycoplasma pneumoniae infection in children. Conclusions The rate of Mycoplasma pneumoniae infection in children needs to be further reduced. Knowing the characteristics and risk factors of Mycoplasma pneumoniae infection in children, effective prevention and control measures can be adopted based on the specific circumstances of the infection and risk factors to minimize its impact on the health of children with pneumonia.

Objective To study the detection and drug resistance of Helicobacter pylori （H. pylori） in patients with chronic gastritis. Methods A total of 2 259 patients with chronic gastritis diagnosed and treated at a hospital in Jinan City from July 2021 to December 2022 were selected for this study. The presence of H. pylori was detected using the ¹³C-urea breath test, and samples were collected from biopsy of the gastric mucosa for culture. The drug-resistant phenotype of H. pylori to metronidazole, levofloxacin, amoxicillin, and clarithromycin was detected in vitro, and the corresponding antibiotic-resistant genes were detected using sequencing methods. Results A total of 839 cases were detected as positive for H. pylori, with a positive detection rate of 37.14%. A total of 758 strains of H. pylori were isolated and cultured, the resistance rates to metronidazole, levofloxacin, amoxicillin, and clarithromycin were 51.98%, 31.13%, 1.00%, and 19.26%, respectively. There was no significant difference in drug resistance rates among H. pylori positive patients of different genders （P>0.05）. However, the drug resistance rate of H. pylori positive patients who had used the above-mentioned drugs was significantly higher than that of those who had not used the corresponding drugs （P<0.05 or P<0.01）. Although no statistically significant difference was observed in the resistance rate to metronidazole and amoxicillin （P>0.05）, there was a statistically significant difference in the resistance rate to levofloxacin and clarithromycin （P<0.05 or P<0.01） in H. pylori positive patients of different ages. The resistance gene of H. pylori to metronidazole was rdxA, and the mutation rates at T184G, G616A, and C148T sites were 70.98%, 43.01%, and 6.86%, respectively. The resistance gene of H. pylori to levofloxacin was gyrA, and the mutation rates at G261G, C261A, G271A, and A272G sites were 7.39%, 5.80%, 7.39%, and 3.96%, respectively. The resistance gene of H. pylori to amoxicillin was pbplA, and the mutation rates at A1777G and C1667G were 10.03% and 4.49%, respectively. The resistance gene of H. pylori to clarithromycin was 23SrRNA, and the mutation rates at T2182C, A2142G, and A2143G were 34.30%, 2.37%, and 20.32%, respectively. Conclusions Patients with chronic gastritis have high rates of H. pylori detection and their drug resistance is not promising. Effective interventions based on the resistance of commonly used antibiotics and the results of related genetic tests should be implemented, and individualized eradication therapies guided by resistance detection should be implemented to promote the eradication of H. pylori.

Objective To explore the compliance of liver cancer screening in high-risk population for liver cancer in the Anhui region, and analyze the related influencing factors, to provide a reference for formulating relevant management strategies. Methods Adult residents who participated in a physical examination in a hospital in Suzhou City from November 2020 to May 2022 and were assessed to be at high risk of liver cancer according to the results of the anti-cancer assessment were selected as the subjects for abdominal ultrasound examination and α-fetoprotein （AFP） detection. A self-made questionnaire was used to investigate the high-risk population for liver cancer. The screening status of the high-risk population for liver cancer was analyzed, and the influencing factors of screening compliance for the high-risk population for liver cancer were analyzed through univariate and multivariate analysis methods. Results Among the 802 high-risk individuals for liver cancer, 386 underwent subsequent liver cancer screening, with a participation rate of 48.13%. Multivariate Logistic regression analysis showed that age < 60 years old （OR=4.424）, Han nationality （OR=2.765）, alcohol consumption （OR=5.551）, chronic hepatitis （OR=4.250）, fatty liver （OR=5.140）, history of gallstones （OR=2.757）, and family history of liver cancer （OR=5.590） were independent influencing factors for compliance of liver cancer screening in high-risk population for liver cancer. Conclusions The participation compliance of liver cancer screening in the high-risk population for liver cancer in the Anhui region is not high. It is of great value to provide certain intervention measures for relevant influencing factors to improve screening compliance.

Objective To study the influencing factors of breastfeeding within six months among postpartum women with delayed lactation initiation. Methods Taking postpartum women with delayed lactation initiation from three hospitals in Dazu District, Chongqing City, from January 2021 to December 2021 for this study. The age, occupation, delivery times, delivery mode, gestational week, nipple flat/sag, suckling frequency, delayed lactation time, postpartum anxiety, postpartum depression, postpartum fatigue, and other conditions of women with delayed lactation initiation were descriptive analyzed. Using WeChat, telephone, and online questionnaires to followed the breastfeeding status of postpartum women for six months and analyzed the influencing factors of continued breastfeeding for postpartum women with delayed lactation initiation through univariate and multivariate analysis methods. Results Among the 1 064 cases of delayed lactation initiation, the majority were ≥35 years old, primipara, and cesarean section, accounting for 62.12%, 66.92% and 56.02%, respectively. The breastfeeding rate within six months after delivery was 20.58% （219/1 064）. There were statistically significant differences in breastfeeding rates within six months after delivery among women with delayed lactation initiation with different ages, delivery times, nipple flat/sag, suckling frequency, delayed lactation time, postpartum anxiety, postpartum depression, postpartum fatigue, and breastfeeding education （P<0.05 or P<0.01）. Multivariate Logistic regression analysis revealed that age （OR=0.394）, delivery times （OR=1.050）, nipple flat/sag （OR=0.409）, suckling frequency （OR=3.904）, delayed lactation time （OR=0.386）, postpartum anxiety （OR=0.561）, postpartum depression （OR=0.692）, postpartum fatigue （OR=0.489）, and breastfeeding education （OR=1.881） were influencing factors of breastfeeding within 6 months after delivery among women with delayed lactation initiation. Conclusions In Dazu District of Chongqing, the breastfeeding rate among postpartum women with delayed lactation initiation needs to be improved, which is caused by multiple factors. Therefore, high-risk groups should be identified and targeted interventions should be strengthened, such as providing breastfeeding guidance, postpartum psychological counseling and early fatigue assessment to improve the breastfeeding rate among women with delayed lactation initiation within six months after delivery, and to promote long-term breastfeeding improvement.