Abstract: ObjectiveTo access the acute oral toxicity and subchronic oral toxicity of Panax notoginseng.MethodsTwenty SD rats (10 males, 10 females) were selected to access the acute oral toxicity of Panax notoginseng, using maximum tolerated dose (MTD) method. Eighty SD rats were randomly divided into negative control group, and low, medium and high dose groups (20 rats in each group, half male and half female), to detect the subchronic oral toxicity of Panax notoginseng. Then, Panax notoginsengat was administered by gavages at doses of 0.000, 0.750, 2.372, and 7.500 g/(kg·BW) for 90 days. The general health status of the rats was observed, the weight and food intake were recorded, and the food utilization rate was calculated. In the final stage, hematologic and serum chemical parameters were tested, the gross anatomy was performed, and organ to body weight ratios were determined. The organs of rats in high dose group and negative control group were examined by histopathology.Results The poisoning and death were not occurred during the 14 days of observation period. Appearance and limbs of both male and female rats were normal. No visible lesions were seen in the autopsy. The MTD of Panx notoginseng in SD rats was greater than 20.25 g/(kg·BW). Abnormal general physiological signs, behavior, appearance, fur and size were not observed in the rats in all the dose groups for the subchronic oral toxicity. Body weight, food consumption rate, hematological and blood biochemistrical parameters, organ weights, organ to body weight ratios, and histopathological examination showed no abnormality.ConclusionBased on this experiment, the adverse effect of Panax notoginseng was not observed. The no observed adverse effect level (NOAEL) of Panax notoginseng was greater than 7.500 g/（kg·BW）.