Abstract: ObjectiveTo explore the effects of compound preparation of anti-depression plant extract on monoamine neurotransmitter of chronic unpredictable mild stress (CUMS) model rats.MethodsCUMS combined with solitary feeding were applied to induce the depression. Three weeks later, the model rats were treated with compound preparation of anti-depression plant extract (144, 288, 576 mg/kg) and imipramine hydrochloride (15 mg/kg) for 8 weeks respectively. Meanwhile, a normal control group was set and fed regularly. During the experiment, the sugar consumption test was conducted regularly. After the treatment, the rats were anesthetized to collect the blood from the abdominal aorta and then sacrificed to dissect and isolate the brain tissues. The contents of NE, DA, 5-HIAA, and 5-HT in brain and plasma were detected by HPLC-FLD. ResultsAfter 3 weeks of modeling, the sugar consumption of model group decreased significantly compared with the normal control group (P＜0.05); after 8 weeks of administration, the consumption of sugar water of model control group was less than that of the other groups (P＜0.05); the levels of monoamine neurotransmitter in brain and plasma of model control group were lower than those of the normal control group and some of the other model groups (P＜0.05).ConclusionThe compound preparation of anti-depression plant extract may improve the depressive behavior of CUMS model rats by increasing the levels of monoamine neurotransmitter.