Abstract: ObjectiveTo understand risk factors of brucellosis among the key occupational groups in Tangshan City, so as to provide a reference basis for prevention and treatment of brucellosis.MethodsA case-control study was conducted. Cases of confirmed brucellosis and cases of silent infection of brucellosis were selected from the occupational population in a screening survey of brucellosis in Tangshan City in 2014 as case group; individuals without infection were selected from the same occupational population at the same period as control group. Questionnaire survey was conducted among the two groups. Univariate and multivariate logistic regression models were used to analyze the data.ResultsA total of 107 cases were investigated in the case group, including 80 males and 27 females, 74.77% of them were at the age of 40-69 years old, and 89.72% had the education level at junior middle school and below. A total of 680 individuals were recruited in the control group, including 463 males and 217 females, 66.32% of them were at the age of 40-69 years old, and 87.50% had the education level at junior middle school and below. There were no significant differences in gender, age and education level between the case group and control group (P>0.05). Multivariate logistic regression analysis showed that contacting with sheep（OR=18.493，95%CI=9.235-37.032）, without using protective clothing or gloves（OR=4.319，95%CI=2.412-7.734）, or Without use of disinfectant（OR=3.024，95%CI=1.861-4.913）, without regular disinfection for feeding environment（OR=4.341，95%CI=2.586-7.288）, handling abortion or delivering sheep（OR=2.437，95%CI=1.564-3.799）, injured skin exposed to animals（OR=2.053，95%CI=1.262-3.340）, intake of dead animals （OR=10.911，95%CI=3.500-34.015), eating undercooked meat（OR=7.714，95%CI=2.908-20.466）, and drinking raw milk（OR=6.673，95%CI=2.111-21.091）were risk factors for brucellosis infection in the key occupational groups. Using protective measures（OR=0.402，95%CI=0.243-0.664） was protective factors for the infection of brucellosis.ConclusionNo effective personal protection measures and unhealthy eating habits were the main risk factors for incidence of brucellosis. It is necessary to strengthen livestock quarantine and market supervision, carry out surveillance, health education and behavior intervention and personal protection in occupational groups to prevent and control brucellosis.