miR-148a-DNMT1对汽油添加剂甲基叔丁基醚所致16HBE细胞DNA损伤的影响

doi:10.12183/j.scjpm.2021.1072

华南预防医学 ›› 2021, Vol. 47 ›› Issue (8): 1072-1075.doi: 10.12183/j.scjpm.2021.1072

miR-148a-DNMT1对汽油添加剂甲基叔丁基醚所致16HBE细胞DNA损伤的影响

贺中汉^1,2, 叶伟国¹, 沙焱³, 方道奎⁴, 胡大林²

1.深圳市宝安区石岩预防保健所,广东深圳 518100;
2.南方医科大学公共卫生学院;
3.深圳市职业病防治院;
4.深圳市疾病预防控制中心

收稿日期:2020-12-01 发布日期:2021-09-14
通讯作者: 胡大林,E-mail：smuhdi@126.com
作者简介:贺中汉（1963—）,男,大学本科,副主任医师,研究方向为职业卫生
基金资助:
生态环境部公益类专项（201009008）; 深圳市科技研发资金项目（JCYJ20170306162947321）

Received:2020-12-01 Published:2021-09-14

摘要/Abstract

摘要： 目的探讨miR-148a-DNMT1 对汽油添加剂甲基叔丁基醚（MTBE）所致人支气管上皮细胞（16HBE）DNA损伤的影响。方法运用Lipofectamine 2000脂质体将miR-148a mimics转染至16HBE细胞;qRT-PCR分析miR-148a及DNMT1 mRNA的表达;Western Blot分析DNMT1蛋白的表达改变;以终浓度为8 µmol/L的MTBE对正常16HBE,16HBE-mimics及16HBE-NC空载体组细胞分别染毒培养48 h;运用单细胞凝胶电泳（SCGE）实验分析各组细胞的DNA损伤程度。结果转染miR-148a mimics的16HBE细胞中miR-148a表达增强（P < 0.05）,DNMT1 mRNA及蛋白表达降低（均P < 0.05）,MTBE对各组细胞DNA有损伤,损伤程度无明显差异（P > 0.05）。结论尚未发现miR-148a-DNMT1的表达变化对汽油添加剂MTBE所致DNA损伤的明显影响。

关键词: 甲基叔丁基醚, DNA损伤, miR-148a, DNA甲基转移酶1, 表观遗传调控

中图分类号:

R113

贺中汉, 叶伟国, 沙焱, 方道奎, 胡大林. miR-148a-DNMT1对汽油添加剂甲基叔丁基醚所致16HBE细胞DNA损伤的影响[J]. 华南预防医学, 2021, 47(8): 1072-1075.

参考文献

[1] Hu D, Yang J, Liu Y, et al.Health risk assessment for inhalation exposure to methyl tertiary butyl ether at petrol stations in southern China[J]. Int J Environ Res Public Health, 2016, 13(2): 204-213.
[2] Yang J, Wei Q, Peng X, et al.Relationship between methyl tertiary butyl ether exposure and non-alcoholic fatty liver disease: A Cross-sectional study among petrol station attendants in southern China[J]. Int J Environ Res Public Health, 2016, 13(10): 946-955.
[3] 叶伟国, 黄浩宇, 姜燃, 等. 汽油添加剂甲基叔丁基醚的职业性暴露对血清丙氨酸转氨酶影响的多因素Logistic回归分析[J]. 毒理学杂志, 2018,32(4):259-263.
[4] Baccarelli A, Bollati V.Epigenetics and environmental chemicals[J]. Curr Opin Pediatr, 2009, 21(2): 243-251.
[5] Sun X, He Y, Huang C, et al.The epigenetic feedback loop between DNA methylation and microRNAs in fibrotic disease with an emphasis on DNA methyltransferases[J]. Cell Signal,2013, 25(9): 1870-1876.
[6] Ji W, Yang L, Yuan J, et al.MicroRNA-152 targets DNA methyltransferase 1 in NiS-transformed cells via a feedback mechanism[J]. Carcinogenesis, 2013, 34(2): 446-453.
[7] Hong L, Sun G, Peng L, et al.The interaction between miR-148a and DNMT1 suppresses cell migration and invasion by reactivating tumor suppressor genes in pancreatic cancer[J]. Oncol Rep, 2018, 40(5): 2916-2925.
[8] 贺中汉,叶伟国,方道奎,等. 汽油添加剂甲基叔丁基醚对DNA聚合酶β低表达的16HBE细胞所致DNA损伤分析[J].毒理学杂志,2020,34(4):296-299,320.
[9] Harvey ZH, Chen Y, Jarosz DF.Protein-based enheritance: epigenetics beyond the chromosome[J]. Mol Cell, 2018, 69(2): 195-202.
[10] Moore-Morris T, van Vliet PP, Andelfinger G, et al. Role of epigenetics in cardiac development and congenital diseases[J]. Physiol rev, 2018, 98(4): 2453-2475.
[11] Sharp GC, Relton CL.Epigenetics and noncommunicable diseases[J]. Epigenomics, 2017, 9(6): 789-791.
[12] Cavalli G, Heard E.Advances in epigenetics link genetics to the environment and disease[J]. Nature, 2019, 571(7766): 489-499.
[13] Hu D, Peng X, Liu Y, et al.Overexpression of miR-221 in peripheral blood lymphocytes in petrol station attendants: A population based cross-sectional study in southern China[J]. Chemosphere, 2016, 149: 8-13.
[14] Lu TX, Rothenberg ME.MicroRNA[J]. J Allergy Clin Immunol, 2018, 141(4): 1202-1207.
[15] Idichi T, Seki N, Kurahara H, et al.Molecular pathogenesis of pancreatic ductal adenocarcinoma: Impact of passenger strand of pre-miR-148a on gene regulation[J]. Cancer Sci, 2018, 109(6): 2013-2026.
[16] Acunzo M, Croce C M.MicroRNA in cancer and cachexia--A mini-review[J]. J Infect Dis, 2015, 212(Suppl 1): S74-S77.
[17] Moore LD, Le T, Fan G.DNA methylation and its basic function[J]. Neuropsychopharmacology, 2013, 38(1): 23-38.
[18] Sriraman A, Debnath TK, Xhemalce B, Miller KM.Making it or breaking it: DNA methylation and genome integrity[J]. Essays Biochem,2020,64(5):687-703.
[19] Sengupta D, et al.Antagonistic activities of miR-148a and DNMT1: Ectopic expression of miR-148a impairs DNMT1 mRNA and dwindle cell proliferation and survival[J]. Gene,2018, 660(20):68-79.
[20] Yang A, Zhang H, Sun Y, et al.Modulation of FABP4 hypomethylation by DNMT1 and its inverse interaction with miR-148a/152 in the placenta of preeclamptic rats and HTR-8 cells[J]. Placenta,2016,46:49-62.
[21] Jackson SP, Bartek J.The DNA-damage response in human biology and disease[J]. Nature, 2009, 461(7267): 1071-1078.

miR-148a-DNMT1对汽油添加剂甲基叔丁基醚所致16HBE细胞DNA损伤的影响

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