应用细胞方法建立表面活性剂眼刺激性分层筛选模型

doi:10.13217/j.scjpm.2015.0318

华南预防医学 ›› 2015, Vol. 41 ›› Issue (8): 318-322.doi: 10.13217/j.scjpm.2015.0318

• 论著 •

应用细胞方法建立表面活性剂眼刺激性分层筛选模型

李庆^1,2，杨颖²，杨杏芬²，杨光宇³，张文改⁴，薛金玉⁵，柯彩纯²

广东省疾病预防控制中心，广东广州 511430

出版日期:2015-08-20 发布日期:2015-10-12
通讯作者: 杨杏芬 E-mail:yangxingfen@21cn.com
作者简介:李庆(1976―)，男，在读硕士研究生，副主任技师，研究方向:应用毒理学

Establishment of a tiered screening model for testing eye irritation of surfactants by in vitro methods

LI Qing^1,2,YANG Yin²,YANG Xing-fen², YANG Guang-yu³,ZHANG Wen-gai⁴ , XUE Jin-yu⁵,KE Cai-chun²

1.School of Public Health,Sun Yat-sen University, Guangzhou 510080, China; 2.Guangdong Provincial Center for Disease Control and Prevention; 3.Guangzhou Center for Disease Control and Prevention;4.Dongguan Center for Disease Control and Prevention; 5.Xiangshan Country Center for Disease Control and Prevention,Zhejiang Province.

Online:2015-08-20 Published:2015-10-12

摘要/Abstract

摘要： 目的建立表面活性剂眼刺激性测试的体外细胞方法分层筛选模型。方法应用中性红摄取试验(NRU)、红细胞溶血试验(RBCH)及荧光素漏出试验(FLT)3种体外细胞方法对20种表面活性剂的眼刺激性进行检测,将检测结果代入4种分层筛选模型(FLT+NRU、FLT+RBCH、RBCH+NRU、NRU+RBCH),所得到的分级结果与Draize试验结果进行比较(指标为Gamma系数、Mc Nemar-Bowker系数、Kappa系数和分级一致率),以此来评价4种分层筛选模型对表面活性剂的检测效能。结果本研究建立的4种分层筛选模型(FLT+NRU、FLT+RBCH、RBCH+NRU、NRU+RBCH)与Draize试验分级结果进行比较,分级一致率分别为90.0%、80.0%、80.0%、65.0%,Kappa值分别为0.84、0.68、0.69、0.47(均P<0.01),Gamma值分别为1.00、1.00、0.93、0.88(均P<0.01)。结论以FLT(第1层)和NRU(第2层)组成的分层筛选模型较其他3种模型具有较好的检测效能,更适合应用于对表面活性剂的眼刺激性检测。

Abstract: ObjectiveTo understand the epidemiological features of human infection with avian influenza virus in Guangdong Province.MethodsDemographic and epidemiologic data on human cases reported during 1998-2014 were collected and analyzed.Epidemiological characteristics and exposure factors of the cases were analyzed by using the method of descriptive epidemiology.ResultsA total of 130 cases infected with avian influenza A viruses were reported from 1998 to 2014, including 4 cases of H5N1, 1 case of H5N6, 1 case of H5 subtype , 113 cases of H7N9,11 cases of H9N2, 43 deaths, with a mortality rate of 33.08%; 81.54% (106/130) of cases occurred from January to March and in December, and 67.69% (88/130) occurred in the Pearl River Delta area.Among all the cases, 81 were male and 49 were female, with a male to female ratio of 1.65∶1; 48.44%（62/128）of cases aged 55 years and over, 92.31% (120/130) had clear exposure to live poultry; 8.33% (10/120) engaged in poultry related occupations, 41.67% (50/120) contacted with poultry, and 50.00% (60/120) had exposure to wet markets with live poultry.ConclusionThe epidemic peaks of human infections with avian influenza virus in Guangdong appeared in winter and spring, with certain regional clusters．The main source of infection was exposure to the live poultry and wet markets with live poultry.

中图分类号:

R994.6

李庆，杨颖，杨杏芬，杨光宇，张文改，薛金玉，柯彩纯. 应用细胞方法建立表面活性剂眼刺激性分层筛选模型[J]. 华南预防医学, 2015, 41(8): 318-322.

LI Qing,YANG Yin,YANG Xing-fen, YANG Guang-yu,ZHANG Wen-gai , XUE Jin-yu,KE Cai-chun. Establishment of a tiered screening model for testing eye irritation of surfactants by in vitro methods[J]. S China J Prev Med, 2015, 41(8): 318-322.

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应用细胞方法建立表面活性剂眼刺激性分层筛选模型

Establishment of a tiered screening model for testing eye irritation of surfactants by in vitro methods

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