关键词: 广东凉茶, 红细胞膜, 氧化损伤, 抗氧化剂, 膜封闭度

Abstract: Objective To observe the protective effect of Guangdong herbal tea extract on oxidative damage of rabbit erythrocyte membrane from the perspective of membrane composition, structure and function. Methods The experiment consisted of a control group, H₂O₂ group （1 000 μmol/L）, 0.1 mg group （0.1 mg/ml herbal tea +1 000 μmol/L H₂0₂）, 0.2 mg group （0.2 mg/mL herbal tea +1 000 μmol/L H₂0₂）, and 0.4 mg group （0.4 mg/mL herbal tea +1 000 μmol/L H₂0₂）. Rabbit red blood cell membrane was prepared by Dodge method. The effects of malondialdehyde （MDA）, the carbonyl content of protein, membrane blocking ability, ATPase activity, glutathione peroxidase （GPX）, catalase （CAT） activity, and membrane protein composition on each group were observed. Results Compared with the control group, the carbonyl content of protein and GPX activity were increased, while cell membrane blocking ability, activity of CAT, Ca²⁺Mg²⁺-ATPase, Mg²⁺-ATPase, and Na⁺K⁺-ATPase in H₂O₂ group were decreased （P < 0.01 for all）. Compared with the H₂O₂ group, contents of MDA and DNPH decreased, while the cell membrane blocking rate, and activities of Ca²⁺Mg²⁺-ATPase, Mg²⁺-ATPase, Na⁺K⁺-ATPase, GPX, and CAT increased in the three herbal tea groups （P < 0.05 for all）. Conclusion Guangdong herbal tea extract could inhibit the damage of erythrocyte membrane composition, structure and function caused by hydrogen peroxide.

Key words: Guangdong herbal tea, Erythrocyte membrane, Oxidative damage, Antioxidant, Blocking degree