关键词: 平卧菊三七, 高尿酸血症, 黄嘌呤氧化酶

Abstract: Objective To investigate the reducing uric acid effect and its mechanism of solid beverage of Gynuraprocumbens （Lour.） Merr. in rats models of hyperuricemia. Methods Sixty male SD rats weighing 180-200 g were randomly divided into 6 groups, including negative control group, hyperuricemia model group, allopurinol 20 mg/（kg·BW） positive control group and solid beverage of Gynuraprocumbens （Lour.） Merr. 1.125, 2.250 and 4.500 g/（kg·BW） groups. The corresponding test substances were given by gavage for 10 days. Serum uric acid （UA）, creatinine （CRE）, urea nitrogen （BUN）, xanthine oxidase （XOD） and liver homogenate XOD levels were measured after 10 days. The mRNA expression levels of renal transporters urate anion transporter 1 （URAT1）, glucose transporter 9 （GLUT9）, sodium-dependent phosphate transporter 1 （NPT1）, ATP binding cassette super family G 2 （ABCG2） were detected by real-time quantitative PCR. Results Solid beverage of Gynuraprocumbens （Lour.） Merr. had no significant effect on body weight in rats. Compared with the hyperuricemia model group, the serum UA levels of rats in the low, medium, and high-dose groups of solid beverage of Gynuraprocumbens （Lour.） Merr. were significantly reduced, and the levels of serum CRE, BUN and XOD and the XOD levels of liver homogenate were also significantly decreased （all P<0.05）. The mRNA expression level of UA salt transporter URAT1 decreased in rats （P<0.05）. Conclusion Solid beverage of Gynuraprocumbens （Lour.） Merr. possesses a potential anti-hyperuricemic effect in rats, and the mechanism is related to decreasing the activity of serum and liver XOD, then interfering the production of UA, and down-regulating the mRNA expression of UA salt transporter URAT1, thereby reducing the reabsorption of UA.

Key words: Gynuraprocumbens （Lour.） Merr., Hyperuricemia, Xanthine oxidase