复合营养素对PM2.5所致大鼠急性肺部氧化应激和炎症损伤的影响

doi:10.12183/j.scjpm.2022.0907

华南预防医学 ›› 2022, Vol. 48 ›› Issue (8): 907-911.doi: 10.12183/j.scjpm.2022.0907

• 论著 • 下一篇

复合营养素对PM_2.5所致大鼠急性肺部氧化应激和炎症损伤的影响

蔡君华¹, 张波¹, 劳文艳², 任超³, 范子轩³

1.南方医科大学公共卫生学院,广东广州 510515;
2.北京联合大学生物化学工程学院;
3.北京联合大学应用文理学院保健食品功能检测中心

收稿日期:2021-10-26 出版日期:2022-08-20 发布日期:2022-10-08
通讯作者: 张波,E-mail：cnzhangbo@126.com;劳文艳,E-mail：laowenyan@buu.edu.cn
作者简介:蔡君华（1989—）,女,硕士研究生,研究方向：营养与食品卫生学

Effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM_2.5exposure in rats

CAI Jun-hua¹, ZHANG Bo¹, LAO Wen-yan², REN Chao³, FAN Zi-xuan³

1. School of Public Health, Southern Medical University, Guangzhou 510515, China;
2. College of Biochemical Engineering, Beijing Union University;
3. Functional Inspection Center of Health Food, College of Applied Science and Humanities, Beijing Union University

Received:2021-10-26 Online:2022-08-20 Published:2022-10-08

摘要/Abstract

摘要： 目的探讨某复合营养素对大气PM_2.5致大鼠急性肺部氧化应激和炎症损伤的预防作用。方法将40只SD大鼠随机分为4组：空白组、PM_2.5模型组、低剂量营养素（150 mg/kg）+PM_2.5组、高剂量营养素（750 mg/kg）+PM_2.5组。营养素组连续预灌胃14 d营养素溶液,空白组、模型组灌胃生理盐水。第15天灌胃后除空白组外的各组大鼠气管滴注PM_2.5（9.0 mg/kg）混悬液染毒,每隔24 h 1次,共3次,空白组气管滴注生理盐水。末次染毒24 h后,收集血样、支气管肺泡灌洗液、肺组织匀浆和病理切片。分析测定血清丙二醛（MDA）、超氧化物歧化酶（SOD）水平,肺泡灌洗液中乳酸脱氢酶（LDH）、酸性磷酸酶（ACP）水平;肺匀浆液中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）水平。结果与空白组比较,PM_2.5模型组的大鼠MDA、ACP、LDH、TNF-α、IL-1β水平增加、SOD活力降低（P<0.05或P<0.01）;与PM_2.5模型组比较,高、低剂量营养素+PM_2.5组能降低MDA、TNF-α、IL-1β水平及升高SOD活力（P<0.05或P<0.01）,高剂量营养素+PM_2.5组能降低ACP、LDH水平（P<0.05或P<0.01）,并改善大鼠肺组织病理变化。结论该复合营养素对PM_2.5致大鼠急性肺部氧化应激及炎症损伤具有保护作用。

关键词: 复合营养素, 肺损伤, PM_2.5, 氧化应激, 炎症损伤

Abstract: Objective To investigate the preventive effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM_2.5 exposure in rats. Methods Forty SD rats were randomized into 4 groups： control group, PM_2.5model group, low-dose supplement （150 mg/kg） + PM_2.5 group, and high-dose supplement （750 mg/kg） + PM_2.5 group. Rats in nutrient supplement groups were administrated continuous by supplement solution gavage for 14 days, while rats in control and model groups were given with saline gavage. On the 15th day, rats in all groups except control group were exposed to PM_2.5 （9.0 mg/kg） suspension by tracheal drip, once every 24 hours, for 3 times in total. Rats in control group was treated with saline as the same procedure. Twenty-four hours after the last exposure, blood samples, bronchoalveolar lavage fluid, lung tissue homogenate and pathological sections of rats were collected. The levels of malondialdehyde （MDA）, superoxide dismutase （SOD） in serum,the levels of lactate dehydrogenase （LDH） and acid phosphatase （ACP） in alveolar lavage fluid and the levels of tumor necrosis factor-α （TNF-α）, interleukin 1β （IL-1β） in lung homogenates were measured. Results Compared with the control group, the levels of MDA, ACP, LDH, TNF-α, IL-1β in PM_2.5model group were significantly higher and SOD activity was lower （P<0.05 or P<0.01）. Compared with the PM_2.5model group, the levels of MDA, TNF-α and IL-1β in nutrient supplement + PM_2.5 groups were significantly lower, and SOD activity was higher （P<0.05 or P<0.01）. Compared with the PM_2.5model group, the levels of ACP and LDH in high-dose supplement + PM_2.5 group were significantly lower （P<0.05 or P<0.01）, and the pathological injury of lung tissues were significantly less severe. Conclusion The composite nutrient supplement exerts protective effect on acute lung oxidative stress and inflammatory injury induced by PM_2.5 exposure in rats.

Key words: Composite nutrient supplement, Lung injury, PM_2.5, Oxidative stress, Inflammatory injury

中图分类号:

R114

蔡君华, 张波, 劳文艳, 任超, 范子轩. 复合营养素对PM_2.5所致大鼠急性肺部氧化应激和炎症损伤的影响[J]. 华南预防医学, 2022, 48(8): 907-911.

CAI Jun-hua, ZHANG Bo, LAO Wen-yan, REN Chao, FAN Zi-xuan. Effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM_2.5exposure in rats[J]. South China Journal of Preventive Medicine, 2022, 48(8): 907-911.

参考文献

[1] Lim CH, Ryu J, Choi Y, et al.Understanding global PM_2.5 concentrations and their drivers in recent decades (1998-2016)[J]. Environ Int, 2020,144:106011.
[2] 樊乃根. PM_2.5对人体健康影响的研究进展[J]. 中国城乡企业卫生, 2018,33(3):55-56.
[3] 吕静,陶文沂,程景才,等. 十字花科蔬菜提取物抗肿瘤作用的研究[J].食品与生物技术学报, 2007(4):105-109.
[4] 韩书芝,李海月,张凤蕊,等.维生素C对PM_2.5染毒大鼠肺组织氧化损伤的影响[J].中国老年学杂志, 2021,41(19):4373-4377.
[5] 李航. PM_2.5对大鼠肺脏的毒性效应及鱼油和维生素E的干预作用[D]. 新乡:新乡医学院, 2017.
[6] 郭淼,闫鹏,韩国鑫,等.N-乙酰半胱氨酸对PM_2.5致支气管上皮细胞损伤的保护作用体外实验[J].解放军医学院学报, 2021,42(1):99-103.
[7] Wang Z, Feng F, He H, et al.Citrus alkaline extracts prevent endoplasmic reticulum stress in type II alveolar epithelial cells to ameliorate pulmonary fibrosis via the ATF3/PINK1 pathway[J]. Phytomedicine, 2021,89:153599.
[8] Jones AW, March DS, Curtis F, et al.Bovine colostrum supplementation and upper respiratory symptoms during exercise training: a systematic review and meta-analysis of randomised controlled trials[J]. BMC Sports Sci Med Rehabil, 2016,8(1):21.
[9] Sese L, Jeny F, Uzunhan Y, et al.The effect of air pollution in diffuse interstitial lung disease[J]. Revue Des Maladies Respiratoires, 2020,37(5):389-398.
[10] 邓翔,张星亮,张玲莉,等. PM_2.5及其对肺组织损伤的研究进展[J].中华实用儿科临床杂志, 2019,34(23):1828-1830.
[11] 段肖肖,刘思秀. PM_2.5致病机制的研究进展[J].复旦学报(医学版), 2020,47(4):605-614.
[12] Li R, Kou X, Xie L, et al.Effects of ambient PM_2.5 on pathological injury, inflammation, oxidative stress, metabolic enzyme activity, and expression of c-fos and c-jun in lungs of rats[J]. Environ Sci Pollut Res Int, 2015,22(24):20167-20176.
[13] Shan X, Liu L, Li G, et al.PM_2.5 and the typical components cause organelle damage, apoptosis and necrosis: Role of reactive oxygen species[J]. Sci Total Environ, 2021,782:146785.
[14] 韩书芝,李海月,尹航,等. PM_2.5对大鼠肺组织Nrf2/HO-1通路的影响及维生素C的干预作用[J].国际呼吸杂志, 2020,40(21):1631-1635.
[15] 李莉珊,马琼锦,杨凌,等.维生素E对PM_2.5急性染毒引起大鼠肺损伤的影响[J].营养学报,2016,38(3):256-260.
[16] Fen P, Qin C, Hua L, et al.Antagonistic effects of N-acetylcysteine on mitogen-activated protein kinase pathway activation, oxidative stress and inflammatory responses in rats with PM_2.5 induced lung[J]. Chin Med Sci J, 2019,34(4):270-276.
[17] Zhou XM, Huang MM, He CC, et al.Inhibitory effects of citrus extracts on the experimental pulmonary fibrosis[J]. J Ethnopharmacology, 2009,126(1):143-148.
[18] Abdull RA, Bagatta M, De Nicola GR, et al.Up-regulation of cytochrome P450 and phase II enzyme systems in rat precision-cut rat lung slices by the intact glucosinolates, glucoraphanin and glucoerucin[J]. Lung Cancer, 2011,71(3):298-305.
[19] Xu ML, Kim HJ, Wi GR, et al.The effect of dietary bovine colostrum on respiratory syncytial virus infection and immune responses following the infection in the mouse[J]. J Microbiol, 2015,53(9):661-666.

复合营养素对PM_2.5所致大鼠急性肺部氧化应激和炎症损伤的影响

Effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM_2.5exposure in rats

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复合营养素对PM2.5所致大鼠急性肺部氧化应激和炎症损伤的影响

Effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM2.5 exposure in rats

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复合营养素对PM_2.5所致大鼠急性肺部氧化应激和炎症损伤的影响

Effect of a composite nutrient supplement on acute lung oxidative stress and inflammatory injury induced by PM_2.5exposure in rats