1995—2024年柯萨奇病毒B组5型广东省分离株基因特征分析

doi:10.12183/j.scjpm.2025.0490

华南预防医学 ›› 2025, Vol. 51 ›› Issue (5): 490-495.doi: 10.12183/j.scjpm.2025.0490

1995—2024年柯萨奇病毒B组5型广东省分离株基因特征分析

陈乐妍^1,2, 陈启方^2,4, 黄玉梅^1,2, 王博俊^2,4, 毛锐坤^1,2, 王乐^2,4, 柯昌文^1,2,3

1.广州中医药大学中药学院,广东广州 510006;
2.广东省疾病预防控制中心;
3.广东省新发传染病应急检测技术研究与应用重点实验室;
4.南方医科大学公共卫生学院

收稿日期:2025-01-01 发布日期:2025-06-27
通讯作者: 柯昌文,E-mail：kecw2011@sina.com
作者简介:陈乐妍（2000—）,女,在读硕士研究生,研究方向：肠道病毒分子流行病学
基金资助:
广东省科技计划项目（2023B1212010010）;广东卫生健康平台科技创新人才项目（0620220104）

Genetic characteristics of Coxsackievirus B5 in Guangdong Province, 1995—2024

CHEN Leyan^1,2, CHEN Qifang^2,4, HUANG Yumei^1,2, WANG Bojun^2,4, MAO Ruikun^1,2, WANG Le^2,4, KE Changwen^1,2,3

1. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China;
2. Guangdong Provincial Center for Disease Control and Prevention;
3. Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response;
4. Southern Medical University School of Public Health

Received:2025-01-01 Published:2025-06-27

摘要/Abstract

摘要： 目的了解1995—2024年广东省急性弛缓性麻痹（acute flaccid paralysis,AFP）病例中分离到的柯萨奇病毒B组5型（coxsackievirus B5,CVB5）分离株的基因特征。方法使用高通量测序对1995—2024年广东省AFP监测分离获得的非脊灰肠道病毒进行分型鉴定,同时结合GenBank公共数据库中收录的CVB5病毒株结构蛋白VP1基因序列,构建系统进化树,研究CVB5基因特征和遗传进化规律。结果从AFP病例样本分离株中鉴定出31株CVB5毒株,并获得其VP1序列;序列全长849 bp,编码283个氨基酸,且未发现氨基酸插入或缺失。31株CVB5毒株VP1序列核苷酸一致性为88.05%（76.10%~100.00%）,氨基酸同源性为97.15%（94.30%~100.00%）;其中B基因型1株,C基因型27株,D基因型3株。基于全球CVB5 VP1区的进化分析显示,1996—2024年亚洲地区多数分离株为C基因型,欧洲地区分离株为D基因型。结论 CVB5在世界多地持续流行,1995—2024年广东省CVB5分离株包含3个基因型且以C基因型为主,与亚洲地区流行基因型相同。D基因型分离株出现表明需继续加强对广东地区CVB病毒的专项监测和研究。

关键词: 柯萨奇病毒B组5型, VP1, 进化分析

Abstract: Objective To understand the genetic characteristics of the Coxsackievirus B5 (CVB5) isolated from cases of acute flaccid paralysis (AFP) in Guangdong Province from 1995 to 2024. Methods High-throughput sequencing was used to identify non-polio enteroviruses isolated from AFP surveillance in Guangdong Province from 1995 to 2024. The VP1 gene sequences of CVB5 strains in GenBank were used to construct a phylogenetic tree and study the genetic evolution of CVB5. Results 31 CVB5 strains were identified from AFP case specimens, and their VP1 sequences were obtained.The full-length VP1 sequence was 849 bp, encoding 283 amino acids, with no insertions or deletions. The nucleotide and amino acid homology of the 31 CVB5 VP1 sequences were 88.05% (76.10%-100.00%) and 97.15% (94.30%-100.00%), respectively. Among them, there was 1 strain of genotype B, 27 strains of genotype C, and 3 strains of genotype D. Evolutionary analysis of the global CVB5 VP1 region showed that most strains isolated in Asia from 1996 to 2024 were genotype C, while strains from Europe were genotype D. Conclusions CVB5 has been circulating in many parts of the world. The CVB5 strains isolated in Guangdong Province from 1995 to 2024 included three genotypes, mainly genotype C, which is the same as the prevalent genotype in Asia. The emergence of genotype D strains indicates the need for continued monitoring and research on CVB5 in Guangdong.

Key words: Coxsackievirus B5, VP1, Evolutionary analysis

中图分类号:

R373.9

陈乐妍, 陈启方, 黄玉梅, 王博俊, 毛锐坤, 王乐, 柯昌文. 1995—2024年柯萨奇病毒B组5型广东省分离株基因特征分析[J]. 华南预防医学, 2025, 51(5): 490-495.

CHEN Leyan, CHEN Qifang, HUANG Yumei, WANG Bojun, MAO Ruikun, WANG Le, KE Changwen. Genetic characteristics of Coxsackievirus B5 in Guangdong Province, 1995—2024[J]. South China Journal of Preventive Medicine, 2025, 51(5): 490-495.

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1995—2024年柯萨奇病毒B组5型广东省分离株基因特征分析

Genetic characteristics of Coxsackievirus B5 in Guangdong Province, 1995—2024

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