广东省肠道病毒B组75型分离株的全基因组特征分析

doi:10.12183/j.scjpm.2025.0496

华南预防医学 ›› 2025, Vol. 51 ›› Issue (5): 496-502.doi: 10.12183/j.scjpm.2025.0496

广东省肠道病毒B组75型分离株的全基因组特征分析

陈启方^1,2, 陈乐妍^2,4, 王博俊^1,2, 黄玉梅^2,4, 王乐^1,2, 毛锐坤^2,4, 张宝¹, 柯昌文^1,2,3

1.南方医科大学公共卫生学院,广东广州 510515;
2.广东省疾病预防控制中心;
3.广东省新发传染病应急检测技术研究与应用重点实验室;
4.广州中医药大学中药学院

收稿日期:2025-01-01 发布日期:2025-06-27
通讯作者: 柯昌文,E-mail：kecw1965@aliyun.com;张宝,E-mail：zhang20051005@126.com
作者简介:陈启方（1998—）,女,在读硕士研究生,主要研究方向：传染病预防与控制
基金资助:
广东省科技计划项目（2023B1212010010）;广东卫生健康平台科技创新人才项目（0620220104）

Whole genome characterization of Enterovirus B75 isolates from Guangdong Province

CHEN Qifang^1,2, CHEN Leyan^2,4, WANG Bojun^1,2, HUANG Yumei^2,4, WANG Le^1,2, MAO Ruikun^2,4, ZHANG Bao¹, KE Changwen^1,2,3

1. School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China;
2. Guangdong Provincial Center for Disease Control and Prevention;
3. Guangdong Provincial Key Laboratory of Pathogen Detection for Emerging Infectious Disease Response;
4. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine

Received:2025-01-01 Published:2025-06-27

摘要/Abstract

摘要： 目的回溯性地对广东省1994—1999年AFP监测系统分离的肠道病毒B组75型（EV-B75）进行全基因组特征及重组分析,为我国新型肠道病毒的分子特征研究补充重要基础数据。方法使用探针捕获富集法对目标基因进高通量测序,获得全基因组序列。基于邻接法构建VP1区及P1~P3编码区系统进化树,分析病毒遗传进化关系;使用Simplot分析验证EV-B75与其他肠道病毒的重组情况。结果 VP1进化树显示,EV-B75总体分为Cluster 1和Cluster 2两大分支;广东分离株与福建、山东及云南分离株同属于Cluster 2,与Cluster 1的原型株及其他地区分离株差异显著,遗传关系较远,具有地域特异性。P1~P3区进化树的聚类差异提示广东株在分子进化中存在重组;Simplot分析证实其在2A~2C、3D区与EV-B组的E9、CVB2/3/4等血清型发生型间重组。结论本研究的7株毒株是广东省早期分离的EV-B75新型肠道病毒,均属于在我国散发流行的Cluster 2分支;其在非结构蛋白区的高频型间重组现象表明EV-B75可通过基因重组产生遗传多样性。本研究对追溯EV-B组病毒的演化历史具有参考意义。

关键词: 肠道病毒B75, 全基因组序列特征, 进化分析, 重组分析

Abstract: Objective To retrospectively delineate the whole-genome features and recombination patterns of Enterovirus group B serotype 75 (EV-B75) strains isolated from the acute flaccid paralysis (AFP) surveillance system in Guangdong Province during 1994–1999, thereby providing foundational molecular data on this emerging enterovirus in China. Methods Target viral RNA was enriched with probe-capture technology followed by high-throughput sequencing to obtain complete genomic sequences. Phylogenetic relationships were inferred using neighbour-joining trees constructed for the VP1 region and the P1-P3 coding regions. Potential recombination events between EV-B75 and other enteroviruses were evaluated and confirmed with SimPlot analyses. Results VP1 phylogenetic analysis classified EV-B75 into two major clusters: Cluster 1 and Cluster 2. All Guangdong isolates grouped with strains from Fujian, Shandong, and Yunnan within Cluster 2, displaying pronounced genetic divergence from the prototype strain and other Cluster 1 isolates, consistent with geographic specificity. Discordant clustering patterns in P1-P3 phylogenetic trees suggested recombination during molecular evolution. SimPlot analyses verified intertypic recombination with EV-B serotypes E9 and CVB2/3/4 in the 2A-2C and 3D regions. Conclusions The seven strains analysed represent the early documented EV-B75 isolates from Guangdong and belong to the sporadically circulating Cluster 2 lineage in China. Frequent intertypic recombination in non-structural protein regions underscores the capacity of EV-B75 to generate genetic diversity via genetic exchange. These findings contribute to reconstructing the evolutionary history of EV-B viruses.

Key words: Enterovirus B75, Whole-genome sequencing, Phylogenetic analysis, Recombination analysis

中图分类号:

R373.2

陈启方, 陈乐妍, 王博俊, 黄玉梅, 王乐, 毛锐坤, 张宝, 柯昌文. 广东省肠道病毒B组75型分离株的全基因组特征分析[J]. 华南预防医学, 2025, 51(5): 496-502.

CHEN Qifang, CHEN Leyan, WANG Bojun, HUANG Yumei, WANG Le, MAO Ruikun, ZHANG Bao, KE Changwen. Whole genome characterization of Enterovirus B75 isolates from Guangdong Province[J]. South China Journal of Preventive Medicine, 2025, 51(5): 496-502.

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广东省肠道病毒B组75型分离株的全基因组特征分析

Whole genome characterization of Enterovirus B75 isolates from Guangdong Province

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