广东省肠道病毒B组75型分离株的全基因组特征分析

doi:10.12183/j.scjpm.2025.0496

Abstract

Abstract: Objective To retrospectively delineate the whole-genome features and recombination patterns of Enterovirus group B serotype 75 (EV-B75) strains isolated from the acute flaccid paralysis (AFP) surveillance system in Guangdong Province during 1994–1999, thereby providing foundational molecular data on this emerging enterovirus in China. Methods Target viral RNA was enriched with probe-capture technology followed by high-throughput sequencing to obtain complete genomic sequences. Phylogenetic relationships were inferred using neighbour-joining trees constructed for the VP1 region and the P1-P3 coding regions. Potential recombination events between EV-B75 and other enteroviruses were evaluated and confirmed with SimPlot analyses. Results VP1 phylogenetic analysis classified EV-B75 into two major clusters: Cluster 1 and Cluster 2. All Guangdong isolates grouped with strains from Fujian, Shandong, and Yunnan within Cluster 2, displaying pronounced genetic divergence from the prototype strain and other Cluster 1 isolates, consistent with geographic specificity. Discordant clustering patterns in P1-P3 phylogenetic trees suggested recombination during molecular evolution. SimPlot analyses verified intertypic recombination with EV-B serotypes E9 and CVB2/3/4 in the 2A-2C and 3D regions. Conclusions The seven strains analysed represent the early documented EV-B75 isolates from Guangdong and belong to the sporadically circulating Cluster 2 lineage in China. Frequent intertypic recombination in non-structural protein regions underscores the capacity of EV-B75 to generate genetic diversity via genetic exchange. These findings contribute to reconstructing the evolutionary history of EV-B viruses.

Key words: Enterovirus B75, Whole-genome sequencing, Phylogenetic analysis, Recombination analysis

CLC Number:

R373.2

CHEN Qifang, CHEN Leyan, WANG Bojun, HUANG Yumei, WANG Le, MAO Ruikun, ZHANG Bao, KE Changwen. Whole genome characterization of Enterovirus B75 isolates from Guangdong Province[J].South China Journal of Preventive Medicine, 2025, 51(5): 496-502.

References

[1] Lefkowitz EJ, Dempsey DM, Hendrickson RC, et al.Virus taxonomy: The database of the International Committee on Taxonomy of Viruses (ICTV)[J]. Nucleic Acids Res, 2018, 46(D1): D708-D717.
[2] Knowles NJ. Picornavirus home page[EB/OL]. (2024-05-20)[2024-12-30]. https://www.picornaviridae.com/.
[3] Brouwer L, Moreni G, Wolthers KC, et al.World-wide prevalence and genotype distribution of enteroviruses[J]. Viruses, 2021, 13(3): 434.
[4] Xie ZF, Khamrin P, Maneekarn N, et al.Epidemiology of enterovirus genotypes in association with human diseases[J]. Viruses, 2024, 16(7): 1165.
[5] Xu JL, Sun ZY, Li W, et al.Epidemiological characteristics and cerebrospinal fluid cytokine profiles of Enterovirus encephalitis in children in Hangzhou, China[J]. J Med Virol, 2022, 94(6): 2645-2652.
[6] Antona D, Kossorotoff M, Schuffenecker I, et al.Severe paediatric conditions linked with EV-A71 and EV-D68, France, May to October 2016[J]. Euro Surveill, 2016, 21(46): 30402.
[7] Simmonds P, Gorbalenya AE, Harvala H, et al.Recommendations for the nomenclature of enteroviruses and rhinoviruses[J]. Arch Virol, 2020, 165(3): 793-797.
[8] Oberste MS, Michele SM, Maher K, et al.Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75[J]. J Gen Virol, 2004, 85(Pt 11): 3205-3212.
[9] Rao CD, Yergolkar P, Shankarappa KS.Antigenic diversity of enteroviruses associated with nonpolio acute flaccid paralysis, India, 2007-2009[J]. Emerg Infect Dis, 2012, 18(11): 1833-1840.
[10] Patil PR, Chitambar SD, Gopalkrishna V.Molecular surveillance of non-polio enterovirus infections in patients with acute gastroenteritis in Western India: 2004-2009[J]. J Med Virol, 2015, 87(1): 154-161.
[11] Kumar A, Shukla D, Kumar R, et al.Molecular epidemiological study of enteroviruses associated with encephalitis in children from India[J]. J Clin Microbiol, 2012, 50(11): 3509-3512.
[12] Kumar A, Shukla D, Kumar R, et al.Environmental surveillance of enterovirus in Northern India using an integrated shell vial culture with a semi-nested RT-PCR and partial sequencing of the VP1 gene[J]. J Clin Microbiol, 2012, 50(11): 3509-3512.
[13] 王海岩,刘昆,陶泽新,等. 肠道病毒B组山东地方株的基因型分布[J]. 病毒学报,2010,26(5):357-362.
[14] Tian BJ, Yoshida H, Wu Y, et al.Molecular typing and epidemiology of non-polio enteroviruses isolated from Yunnan Province, the People's Republic of China[J]. J Med Virol, 2008, 80(4): 670-679.
[15] Zhang KY, Hong M, Zhang Y, et al.Molecular epidemiological, serological, and pathogenic analysis of EV-B75 associated with acute flaccid paralysis cases in Tibet, China[J]. Front Microbiol, 2021, 11: 632552.
[16] Sadeuh-Mba SA, Kavunga-Membo H, Joffret ML, et al.Genetic landscape and macro-evolution of co-circulating coxsackieviruses A and vaccine-derived polioviruses in the Democratic Republic of Congo, 2008-2013[J]. PLoS Negl Trop Dis, 2019, 13(4): e0007335.
[17] Sadeuh-Mba SA, Bessaud M, Massenet D, et al.High frequency and diversity of species C enteroviruses in Cameroon and neighboring countries[J]. J Clin Microbiol, 2013, 51(3): 759-770.
[18] Opanda SM, Wamunyokoli F, Khamadi S, et al.Genotyping of enteroviruses isolated in Kenya from pediatric patients using partial VP1 region[J]. Springerplus, 2016, 5: 158.
[19] Blomqvist S, Paananen A, Savolainen-Kopra C, et al.Eight years of experience with molecular identification of human enteroviruses[J]. J Clin Microbiol, 2008, 46(7): 2410-2413.
[20] Zhu PY, Ji WQ, Li D, et al.Current status of hand-foot-and-mouth disease[J]. J Biomed Sci, 2023, 30(1): 15.
[21] Andrés C, Vila J, Creus-Costa A, et al.Enterovirus D68 in hospitalized children, Barcelona, Spain, 2014-2021[J]. Emerg Infect Dis, 2022, 28(7): 1327-1331.
[22] 徐爱强. 新型人类肠道病毒山东分离株的分子流行病学及其基因组特征研究[D]. 济南:山东大学,2012.
[23] 陶泽新,王海岩,徐爱强,等. 人类肠道病毒B组73、75和97型山东地方株的鉴定及其基因特征分析[J]. 病毒学报,2010,26(1):16-19.
[24] Avellón A, Rubio G, Palacios G, et al.Enterovirus 75 and aseptic meningitis, Spain, 2005[J]. Emerg Infect Dis, 2006, 12(10): 1609-1611.
[25] Lukashev AN, Shumilina EY, Belalov IS, et al.Recombination strategies and evolutionary dynamics of the human enterovirus A global gene pool[J]. J Gen Virol, 2014, 95(Pt 4): 868-873.
[26] Zhang T, Du J, Xue Y, et al.Epidemics and frequent recombination within species in outbreaks of human enterovirus B-associated hand, foot and mouth disease in Shandong China in 2010 and 2011[J]. PLoS One, 2013, 8(6): e67157.
[27] Paananen A, Ylipaasto P, Smura T, et al.A single amino acid substitution in viral VP1 protein alters the lytic potential of clone-derived variants of echovirus 9 DM strain in human pancreatic islets[J]. J Med Virol, 2013, 85(7): 1267-1273.
[28] Dotta F, Censini S, van Halteren AG, et al. Coxsackie B4 virus infection of beta cells and natural killer cell insulitis in recent-onset type 1 diabetic patients[J]. Proc Natl Acad Sci U S A, 2007, 104(12): 5115-5120.
[29] Simmonds P, Welch J.Frequency and dynamics of recombination within different species of human enteroviruses[J]. J Virol, 2006, 80(1): 483-493.
[30] 李晓涵,杨佳新,唐煜斌,等. 人类肠道病毒的研究进展[J]. 生物医学转化,2024,5(1):12-27.
[31] Khetsuriani N, Lamonte-Fowlkes A, Oberst S, et al.Enterovirus surveillance—United States, 1970-2005[J]. MMWR Surveill Summ, 2006, 55(8): 1-20.
[32] Mcwilliam Leitch EC, Cabrerizo M, Cardosa J, et al.Evolutionary dynamics and temporal/geographical correlates of recombination in the human enterovirus echovirus types 9, 11, and 30[J]. J Virol, 2010, 84(18): 9292-9300.
[33] Fratty IS, Kriger O, Weiss L, et al.Molecular analysis of coxsackievirus B2 associated with severe symptoms of the central nervous system[J]. J Med Virol, 2024, 96(11): e70066.
[34] Nekoua MP, Alidjinou EK, Hober D.Persistent coxsackievirus B infection and pathogenesis of type 1 diabetes mellitus[J]. Nat Rev Endocrinol, 2022, 18(8): 503-516.

Whole genome characterization of Enterovirus B75 isolates from Guangdong Province

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Metrics

Comments

Recommended 0