Abstract: Objective To investigate the microbial contamination levels and the contents of metals, fats, and trans fatty acids in ready-to-drink coffee beverages sold in Yuexiu District, Guangzhou, and to provide references for regulatory oversight. Methods A total of 30 ready-to-drink coffee beverage samples were collected and tested for total bacterial count, coliforms, Staphylococcus aureus, Salmonella, Bacillus cereus, Listeria monocytogenes, mold, ochratoxin A, fats, trans fatty acids, and metal contents (lead, chromium, cadmium, total mercury, total arsenic, and aluminum). Results The overall exceedance rate of reference values in the samples was 20% (6/30), with exceedances observed in total bacterial count, coliforms, and molds. Metals were detected at varying levels but all at low concentrations. Trans fatty acid content was ≤0.3 mg/100 g in all samples. The average fat content was 1.9 g/100mL, with higher levels found in coconut latte, cheese/cream latte, and thick milk latte. Conclusion sThe microbial contamination levels in ready-to-drink coffee beverages in Yuexiu District, Guangzhou, in 2024 were controllable. The contents of metals and trans fatty acids were low, posing minimal health risks. However, the additional fat intake from consuming such beverages and its associated health risks should be noted.

Key words: Coffee, Indicator microorganisms, Metals, Fats, Trans fatty acids, Health risk assessment