Abstract: Objective To investigate epidemiological characteristics of hepatitis B in Guangxi and thus explore strategies and measures for prevention and control. Methods The data of hepatitis B from 2005 to 2012 were from the information system of China CDC and analyzed by descriptive epidemiological method. Results A total of 307 302 cases of hepatitis B were reported during 2005 to 2012. The annual report incidence rates were 64.12/100 000 (29 637 cases), 73.64/100 000 (34 317), 73.12/100 000 (34 507), 78.31/100 000 (37 337), 85.21/100 000 (41 035), 85.29/100 000(41 418), 95.81/100 000 (44 099), and 96.77/100 000 (44 952), respectively, with average annual in cidence rate of 81.54/100 000. The annual report incidence rate decreased from 17.52/100 000 (1 752 cases) in 2005 to 7.02/10 0 000 (713) in 2012 in 0-14 age group. Annual report incidence rates showed rising trend in other age groups. The cases were mainly concentrated in 20-39 year group (50.16%,154 132/307 302). The sex ratio was 2.27∶1 (213 321 males∶93 981 females). Peasants accounted for 59.21%（181 946/307 302）of the total cases of hepatitis B.The annual average incidence rates of top three cities were Fangchengang (137.63/100 000), Beihai(94.70/100 000) , and Baise (91.37/100 000), and the lowest one was Qinzhou (45.42/100 000). Among 14 cities, the annual incidence rates were declining in Liuzhou, relatively stable in Beihai, but increasing in other 12 cities. The seasonality was not obvious. First dose of hepatitis B vaccination rates of newborns were 97.71% (686 200/702 300) in 2005 and 99.30% (738 871/744 080) in 2012. Conclusion Annual incidence rates of hepatitis B showed declining trend in children while rising trend in adults in Guangxi. Comprehensive preventive methods including strengthening hepatitis B surveillance and practicing hepatitis B immunization in adults should be the future focus.