Abstract: ObjectiveTo explore depression and its influencing factors among people living with HIV/AIDS (PLWHA) with different genders in Guangzhou. MethodsPLWHA in Guangzhou No.8 Hospital were recruited using convenient sampling method from March to May 2013 and interviewed using a self designed questionnaire that included questions about socio demographic characteristics, HIV infection related factors, depression, social support and self efficacy, and HIV/AIDS related stigma and discrimination. Chi square test and multivariate unconditional logistic regression were used to analyze depression status and its influencing factors.Results A total of 409 PLWHA were included in this study, including 286 males (69.9%) and 123 females (30.1%), and the average age was (36.26±8.82) years. The average scores of depression were （16.13±10.97） in male PLWHA and （16.15±12.71） in female. The prevalence of depression was 49.0% (140/286) in male and 46.3% (57/123) in female (P＞0.05). Multivariate logistic regression analysis indicated that self efficacy (OR: 0.232), HIV related stigma (OR: 3.082) and interaction between gender and social support (OR: 0.663) were significantly associated with depression. In a gender stratified analysis, HIV related stigma (OR: 3.102) and level of self efficacy (OR: 0.176) were associated with depression among male PLWHA, whereas HIV related stigma (OR: 4.309), level of social support (OR: 0.333) and work experience outside hometown (OR: 0.363) were associated with depression among female PLWHA. ConclusionThe prevalence of depression among PLWHA in Guangzhou was relatively high compared with the national average level among the general population. Besides HIV related stigma, important influencing factors of depression among PLWHA were self efficacy for male and social support for female.