Abstract: Objective To analyze the HIV/AIDS related knowledge, behavior, infection status of HIV, and acceptance of HIV/AIDS related services of men who have sex with men (MSM) in Foshan, so as to provide reference for integrated control and prevention. Methods HIV/AIDS sentinel surveillance data of MSM were collected from 2009 to 2013 and analyzed using descriptive epidemiological methods. Results A total of 1 522 MSM were monitored. The awareness rate of HIV/AIDS related knowledge was 85.1%（1 295/1 522）. In the past six months, 81.1%（1 235/1 522）of the MSM had anal sex and 45.0%（556/1 235）of them used condom each time. The total HIV antibody positive rate was 10.4% (159/1 522) and appeared a rising trend（P<0.01）. The HIV antibody positive rate increased from 2.9% in 2009 to 12.7% in 2013, increased by 337.9%. Single factor analysis showed that HIV antibody positive rates were related with the age, degree of education, frequency of condom use, history of drug abuse, history of sexually transmitted diseases, acceptance of condom use promotion education, and HIV/AIDS counseling and testing (P<0.05 or P<0.01). Conclusion The condom using rate among MSM in Foshan City is low and HIV prevalence showed a rising trend. The condom use should be vigorously promoted to reduce the HIV infection among MSM.