Abstract: Objective To evaluate the effect of hand hygiene intervention on kindergartens children in Changping Town, and to provide reference for hand hygiene promotion in kindergartens. Methods In 2019, stratified random sampling method was adopted to select 1 kindergarten with low, medium and high absenteeism rate, respectively, and two rounds of intervention were carried out for the children in the kindergartens. Wilcoxon rank sum test and Chi-square test were used to compare the parents' hand hygiene cognitive behaviors and children's hand hygiene behaviors before and after the first round of intervention, and the children's absence due to infection symptoms before and after the second round of intervention, evaluate intervention effect. Results A total of 338 parents' questionnaires were distributed in the baseline survey, and 328 valid questionnaires were collected after the intervention. There were no significant differences in age, gender and education level of parents before and after the intervention （all P>0.05）. There were 511 children in the baseline survey and 544 children after the intervention. There were no significant differences in gender and household registration before and after the intervention （both P>0.05）. After the first round of intervention, the scores of parents' hand hygiene cognitive behaviors and children's hand hygiene behaviors increased from 2.18 and 2.22 to 2.35 and 2.39, respectively（both P<0.01）. After the second round of intervention, the total absence rate of kindergartens with high absenteeism rate, the absence rate of respiratory symptoms in 3 kindergartens in small and middle classes decreased from 7.09%, 7.58% and 3.01% to 3.01%, 4.38% and 2.23%, respectively（all P<0.01）. Conclusion Intervention measures can promote children to establish good hand hygiene habits, effectively improve children's hand hygiene status and reduce absenteeism due to respiratory diseases.

Key words: Hand hygiene, Intervention, Kindergarten children, Absenteeism