Abstract: Objective To understand the effect of establishing demonstration community of national campaign on healthy lifestyle for all on the knowledge related to lifestyles among community residents in Nanshan District, Shenzhen. Methods A three-month national demonstration community activity was carried out during May to July in 2012. The content of the activity included organizational management, environmental construction, and effect assessment. Simple random sampling method was used to choose residents aged 8 years and over to conduct face to face on-site questionnaires about health knowledge condition before and after the national demonstration community activity. The questionnaire had 10 questions related to healthy lifestyle, oil and salt restriction, reasonable exercise, and healthy body weight. Results Valid questionnaires were obtained from 1 534 participants (729 males and 805 females, averagely aged 40 years) and 1 272 (619 males and 653 females, averagely aged 43 years) before and after the national demonstration community activity, respectively. The gender and age of the two groups were comparable (P＞0.05). After the activity, the awareness rate of healthy lifestyle among the residents raised from 41.17% (6 316/15 340) to 57.94% (7 370/12 720)( P<0.01). The total awareness rates of female and male (60.17% and 55.59%, respectively) after the activity were both significantly higher than those (42.12%, 40.12%) before the activity. The total awareness rates among residents at age groups of 0-18,19-59, and 60 years and over after the activity were 55.00%, 57.15%, and 68.70%, respectively, higher than those at the same age groups before the activity (19.00%, 42.45%, and 36.74%) (all P<0.01 ). Conclusion The rate of awareness related to healthy lifestyles among community residents has been raised greatly after establishing the demonstration community of national campaign on healthy lifestyle for all. Publicity and education on health knowledge should be strengthen for residents aged 18 years and younger.