Abstract: Objective To research the regulation function of EBV-miRNA-BHRF1-1 on p53 expression in nasopharyngeal carcinoma cells, and to explore its application value in the treatment of nasopharyngeal carcinoma. Methods Plasmid expressing EBV-miRNA-BHRF1-1 and empty plasmid vector were used to transfect into nasopharyngeal carcinoma cells as BHRF1-1 group and empty plasmid group, and set the CNE-2 cells join in PBS as PBS group(control group). Then, p53gene mRNA level was detected by RT-PCR method and p53 protein expression level, by Western method, and the growth of nasopharyngeal carcinoma cells was examined by CCK8 colorimetric method. Results BHRF1-1 expression in BHRF1-1 group was (0.98±0.05), higher than that in empty plasmid group (0.66±0.10) and PBS group (0.65±0.12) (both P<0.01). The expression of p53 mRNA in cells in BHRF1-1 group, empty plasmid group, and PBS group were （0.65±0.07）,（0.98±0.06）, and（0.99±0.03）, respectively. The p53mRNA and p53 Protein expression level in BHRF1-1 group were lower than the other two groups (both P<0.01). The cellular proliferation inhibition rate in BHRF1-1 group was 14.9%, higher than that in empty plasmid group (11.2%) and PBS group (2.5%) (both P<0.01). Conclusion EBV-miRNA-BHRF1-1 can effectively control the mRNA level and protein translation level of p53 gene and may have some inhibition effects on nasopharyngeal carcinoma cell proliferation.