寨卡病毒感染人神经母细胞瘤细胞所诱导的细胞因子表达水平变化

doi:10.12183/j.scjpm.2020.0125

华南预防医学 ›› 2020, Vol. 46 ›› Issue (2): 125-128.doi: 10.12183/j.scjpm.2020.0125

寨卡病毒感染人神经母细胞瘤细胞所诱导的细胞因子表达水平变化

陈敬鸿^{1, 2}, 钟秀珠¹, 陈婉¹, 柯昌文³, 焦晓阳²

1.汕头市疾病预防控制中心,广东汕头 515041;
2.汕头大学医学院;
3.广东省疾病预防控制中心

收稿日期:2019-09-03 出版日期:2020-04-20 发布日期:2020-05-22
通讯作者: 焦晓阳,E-mail: xyjiao@stu.edu.cn
作者简介:陈敬鸿（1982—）,男,硕士研究生,副主任技师,从事病原微生物研究工作
基金资助:
汕头市医疗科技计划项目（170905101930782）; 广东省省级科技计划项目（No.2016A020250001）

Changes of cytokine expression levels in human neuroblastoma cells infected with Zika virus

CHEN Jing-hong¹, ZHONG Xiu-zhu¹, CHEN Wan¹, KE Chang-wen³, JIAO Xiao-yang²

1.Shantou Center for Disease Control and Prevention,Shantou 515041,China;
2.Shantou University Medical College;
3.Guangdong Provincial Center for Disease Control and Prevention

Received:2019-09-03 Online:2020-04-20 Published:2020-05-22

摘要/Abstract

摘要： 目的研究寨卡病毒（ZIKV）感染人神经母细胞瘤细胞（SH-SY5Y）所导致的细胞因子分泌的变化,探讨ZIKV影响神经系统功能紊乱的机制。方法建立ZIKV感染SH-SY5Y细胞模型,采用噻唑蓝（MTT）分析法检测细胞活力,采用悬浮芯片系统（Bio-Plex^® 200）Luminex多重检测技术同时定量检测24、48、72 h细胞培养上清液中细胞因子IL-1β、IL-2、IL-4、IL-5、IL-13、IL-18、IFN-γ、TNF-α、IL-12P70、GM-CSF的浓度。结果病毒感染组24、48、72 h细胞存活率均低于正常对照组（均P<0.01）。病毒感染组（24~72 h）分泌IL-1β、IL-5、IFN-γ、TNF-α、IL-12P70、GM-CSF的浓度水平均高于对照组（均P<0.01）。病毒感染组（48 h）分泌的IL-1β、IL-12P70、GM-CSF浓度水平均高于对照组（48 h）,病毒感染组（72 h）分泌TNF-α高于对照组（72h）（P<0.05或P<0.01）。结论 ZIKV感染SH-SY5Y细胞后致其细胞存活率降低,并以时间依赖的方式分泌浓度水平相对较高的促炎性细胞因子。

关键词: 寨卡病毒, 人神经母细胞瘤细胞, 细胞因子

Abstract: Objective To observe changes in cytokine secretion induced by Zika virus （ZIKV） infection in human neuroblastoma SH-SY5Y and explore the mechanism of ZIKV affecting nervous system dysfunction. Methods A model of SH-SY5Y cells infected with ZIKV was established and the cell viability was detected by MTT assay. Cell culture supernatants were harvested at 24, 48, and 72 hours. Concentrations of IL-1β, IL-2, IL-4, IL-5, IL-13, IL-18, IFN-γ, TNF-α, IL-12P70, and GM-CSF were measured simultaneously and quantitatively using the Bio-Plex^? 200 system. Results The cell survival rates at 24, 48, and 72 hours in the virus infection group were lower than those in the normal control group （all for P <0.01）. The levels of IL-1β, IL-5, IFN-γ, TNF-α, IL-12P70, and GM-CSF secreted by the virus infection group （24-72 h） were higher than those of the control group （all for P <0.01）. The levels of IL-1β, IL-12P70, and GM-CSF secreted by the virus infection group （48 h） were higher than those of the control group （48 h）, and the level of TNF-α secreted by the virus infection group （72 h） was higher than that of the control group （72 h）（P <0.05 or P <0.01）. Conclusion SH-SY5Y cells infected with ZIKV resulted in the decrease in cell viability and the secretion of relatively high concentration of pro-inflammatory cytokines in a time-dependent manner.

Key words: Zika virus, Human neuroblastoma cell, Cytokines

中图分类号:

R183.5

陈敬鸿, 钟秀珠, 陈婉, 柯昌文, 焦晓阳. 寨卡病毒感染人神经母细胞瘤细胞所诱导的细胞因子表达水平变化[J]. 华南预防医学, 2020, 46(2): 125-128.

CHEN Jing-hong, ZHONG Xiu-zhu, CHEN Wan, KE Chang-wen, JIAO Xiao-yang. Changes of cytokine expression levels in human neuroblastoma cells infected with Zika virus[J]. South China Journal of Preventive Medicine, 2020, 46(2): 125-128.

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寨卡病毒感染人神经母细胞瘤细胞所诱导的细胞因子表达水平变化

Changes of cytokine expression levels in human neuroblastoma cells infected with Zika virus

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