Abstract: Objective To investigate the contamination level of aflatoxin B₁ in loose-packed peanut oil extracted by a native method in individual workshops in Guangzhou from 2016 to 2021, and to evaluate the corresponding health risk and liver cancer risk of loose-packed peanut oil consumers. Methods In 2016-2021, a total of 203 loose-packed peanut oil extracted by a native method in individual workshops were collected in Guangzhou. Aflatoxin B₁ concentrations were examined by HPLC-fluorometry and HPLC-post-chromatographic derivatization photometry, and evaluated according to the National Food Safety Standard-Limit of Mycotoxins in Food （GB 2761）. Based on the probability assessment theory, methods of margin of exposure （MOE） and mathematical model were used to evaluate the health risk and liver cancer risk of loose-packed peanut oil consumers. Results The detection rate and over-standard rate of aflatoxin B₁ in 203 loose-packed peanut oil were 76.85% （156/203） and 9.85% （20/203）, respectively. The mean and maximum detection values in 2019-2021 were lower than those in 2016-2018. The results of the health risk assessment showed that the MOE values of peanut oil consumers of all ages were bellowed 10 000, and the risk of liver cancer caused by aflatoxin B₁ in high consumption groups was greater than 1 cancer / （1 million people/year）.Conclusions The contamination level of aflatoxin B₁ in loose-packed peanut oil extracted by a native method in individual workshops in Guangzhou has been improved. While the health risk of peanut oil consumers exposed to aflatoxin B₁ in peanut oil still deserves attention. It is suggested to further strengthen the supervision of individual workshops on peanut oil.

Key words: Peanut oil extracted by native method, Aflatoxin B₁, Specialty food, Exposure assessment