Abstract: ObjectiveTo explore the acute oral toxicity and 30-day toxicity of Wanglaoji herbal tea, so as to provide scientific basis for evaluating its edible safety.MethodsAcute oral toxicity test：Wanglaoji herbal tea extract was administered to the NIH mice via oral gavage at doses of 15.0 g/(kg?BW)［equivalent to herbal crude drugs 99.9 g/(kg?BW）］，and symptoms and deaths of NIH mice were observed for 14 days. Thirty-day oral toxicity test: Wanglaoji herbal tea extract was administered to the male and female Sprague Dawley rats via oral gavage at doses of 1.88, 3.75, and 7.50 g/(kg?BW)［equivalent to herbal crude drugs 12.5, 25.0, and 50.0 g/(kg?BW)］, and the same volume of purified water was given to rats in control group, for 30 consecutive days. Hematologic and biochemistry tests were performed, and organs were weighed at the end of the experiment. Histopathological examination was conducted in the control group and 7.50 g/(kg?BW) group.ResultsIn the acute oral toxicity test, no adverse reactions or deaths were observed during the observation period. The LD50 of the acute toxicity was above 15.0 g/(kg?BW). In the 30-day oral toxicity, all dose groups had no obvious effect on body weight, weight gain, food intake, food utilization rate, hematologic tests, biochemical tests, organ weight and the ratios of organs in male and female rats. Histopathological examination did not show any changes associated withWang Laoji herbal tea extract.ConclusionWanglaoji herbal tea extract belongs to non-toxic level, and 7.50 g/(kg?BW) ［equivalent to herbal crude drugs 50.0 g/(kg?BW)］ Wanglaoji herbal tea extract would not cause obvious toxic reaction in rats.