Abstract: Objective To analyze the current situation of multi-drug resistant bacteria （MDRO） infection in operating room of hospital, and to find out its related factors. Methods A total of 12 619 adult patients in the operating room of a hospital in Luzhou from February 2020 to January 2021 were selected as the research object. The data of patients were investigated, the pathogen detection and bacterial drug sensitivity test were carried out, the incidence of MDRO infection in the operating room was counted, and the strains, department distribution, drug resistance and related factors of MDRO infection were analyzed. Results The incidence rate of MDRO infection in the operating room was 2.10%. The major departments with MDRO infection were general surgery （34.28%）, orthopedics （23.78%） and urology surgery （15.22%）. The common strains of MDRO infection in operating room were Acinetobacter baumannii （46.99%）, Staphylococcus aureus （19.91%） and Pseudomonas aeruginosa （12.04%）. Acinetobacter baumannii was completely resistant to ceftriaxone, the resistance rates to ampicillin, ciprofloxacin, ceftazidime, cefepime, gentamicin and tobramycin exceeded 80.00%, and the resistance rate to levofloxacin was less than 30.00%. Staphylococcus aureus was completely resistant to penicillin and oxacillin, the resistance rates to erythromycin and clindamycin exceeded 80.00%. Pseudomonas aeruginosa was completely resistant to ceftriaxone and ampicillin, the resistance rates to ceftazidime, ciprofloxacin and compound sulfamethoxazole exceeded 80.00%, and the resistance rate to amikacin was less than 30.00%. Logistic regression analysis showed that admission to ICU （OR=5.943）, invasive operation （OR=2.704）, long duration of antibiotic use （OR=2.244）, and diabetes mellitus （OR=1.955） were the influencing factors of MDRO infection in operation room. Conclusion The incidence of MDRO infection in the operation room of a hospital in Luzhou is high, which may be affected by admission to ICU, invasive operation, long duration of antibiotic use and diabetes mellitus.

Key words: Multi-drug resistant bacteria, Drug resistance, Bacterial infection