Abstract: Objective To investigate contamination levels of several food pathogens in infant food in Guangdong. Methods According to the population distribution, characteristics of food production and regional distribution features of Guangdong Province, food samples were collected from supermarkets, department stores, convenience stores and farmer's markets in counties, towns and districts of 13 prefecture level cities of the province. Enterobacter sakazakii, Bacillus cereus and Staphylococcus aurea in infant food (including infant formula milk powder and cereal based supplementary food) were detected quantitatively and qualitatively according to the Work Manual for National Food Pathogens Surveillance (Version 2012) . Results A total of 314 samples of infant food were collected from 64 manufacturers, of which, 92 were infant formula milk powder and 222 were cereal based supplementary food. Ten Enterobacter sakazakii strains were confirmed in 314 samples with a detection positive rate of 3.18%, of which 2 strains were from infant formula milk powder (2.17%) and 8 strains from cereal based supplementary food (3.60%). Ninety Bacillus cereus isolates were confirmed and the detection positive rate was 28.67%, of which, 28 strains were from infant formula milk powder (30.43%) and 62 from cereal based supplementary food (27.92%). No Staphylococcus aurea strains were found. The result of quantitative determination showed that one sample was greater than 110 MPN/100g and the others were less than 5 MPN/100g for Enterobacter sakazakii, and 3 samples were greater than 103 MPN/100g and 41 samples less than 102 MPN/100g for Bacillus cereus. Plate count was also used to determine the number of Bacillus cereus in samples. Of 24 samples, it is found that the number of Bacillus cereus in 14 samples were less than 102 CPU/100g, but no Bacillus cereus was observed in other 10 samples. Conclusion The contamination of the Enterobacter sakazakii and Bacillus cereus in infant food should not be ignored. They were potential risks for infant health. The supervision of pathogenic microorganisms in infant food and the surveillance of the related foodborne diseases should be strengthened.