Objective To investigate the anxiety and depression of adolescents during home quarantine of COVID-19, and explore their influencing factors. Methods Using a cluster sampling method, a questionnaire survey of anxiety and depression was conducted among students in 6 middle schools in Shandong, Shannxi, Liaoning, Henan and Fujian Province based on the class. Chi-square test and multivariate Logistic regression were used to analyze the related factors that affect adolescents’ anxiety and depression. Results A total of 11 430 adolescents were included as the research subjects, of which 5 818 were boys （50.9%） and 5 612 were girls （49.1%）;5 503 （48.1%） in rural areas, 5 927 （51.9%） in urban areas; 238 （2.1%） had found COVID-19 cases in their communities or villages, 9 703 （84.9%） were absent, and 1 489 （13.0%） were unknown. A total of 2 706 cases （23.6%） with anxiety and 4 334 cases （37.9%） with depression were detected. Multivariate Logistic regression showed that adolescents who were female （OR=1.673）, older （OR=1.271,1.460）, lower class ranking （OR=1.193,1.575,1.699）, had COVID-19 cases in or unknown their communities or villages （OR=2.320,2.197）, lived in Shandong Province （OR=1.806）, Fujian Province （OR=2.036）, Henan Province （OR=2.406）, Shaanxi Province （OR=1.575） were more likely to have anxiety. Adolescents who were female （OR=1.577）, older （OR=1.306,1.545）, lower class ranking （OR=1.157,1.384,1.527）, had COVID-19 cases or unknown in their communities or villages （OR=2.492,2.329）, lived in Shandong Province （OR=1.876）, Fujian Province （OR=2.335）, Henan Province （OR=2.413）, Shaanxi Province （OR=1.462） were at higher risk of depression. Conclusion During the home quarantine, the detection rate of anxiety and depression among adolescents were relatively high. The education department should conduct mental health education for adolescents in a timely manner.

Objective To analyze the short-term effect of air temperature on the incidence of hand-foot-mouth disease （HFMD） in children in Tianjin from 2014 to 2018. Methods By collecting the data of daily incidence of HFMD, meteorological and environmental pollution factors, and using the distribution lag nonlinear model of time series, the model was screened on the basis of adjusting the potential confounding factors, and the relative risk （RR） was used as the index to estimate the impact of temperature on the incidence of HFMD in children. Results There were 70 027 children aged 0-15 years old with HFMD, including 41 561 boys and 28 466 girls. The sex ratio was 1.46：1. The overall cumulative response curve between daily mean temperature and incidence of HFMD within 16 days of lag time showed an approximate “m” shape, reaching a peak at 25.6 ℃, with RR=1.45 （95% CI： 1.21-1.73）. The temperature at 26.1 ℃ （RR=1.60, 95% CI： 1.26-2.03） in the girl group and 25.9 ℃ （RR=1.60, 95% CI： 1.28-1.99） in the scattered group were more associated with HFMD. When the lag time was 16 days, extreme low temperature environment（-6.3 ℃） had a certain protective effect on HFMD （RR=0.49, 95% CI： 0.28-0.87）, and high temperature environment （24.7 ℃, 31.8 ℃） had a certain risk effect on HFMD （all RR>1, P<0.05）. Conclusion The correlation between daily mean temperature and HFMD is non-linear, and the attention should be paid to girls and scattered children.

Objective To investigate the dietary nutrition status of vocational college students and its relationships with their mental work ability and learning fatigue. Methods The dietary nutrition status of vocational college students were investigated by 24 h dietary retrospective method lasted for 3 days, and the dietary nutrient intake of vocational college students reaching recommended nutrient intake （RNI） were statistically analyzed. The mental work ability index of students with different nutrient intake and the incidence of learning fatigue were compared. Results The average intake of nicotinic acid and vitamin C were less than 80% RNI, while the average intake of energy, fat, protein, vitamin B₁, vitamin B₂, calcium, iron, zinc and selenium ranged from 80% RNI to 120% RNI, but the average intake of vitamin E, phosphorus and sodium were more than 120% RNI. The mental work ability index after class 8 of the students with adequate intake of protein, nicotinic acid, vitamin B₁, vitamin B₂, vitamin C and calcium were higher than those with insufficient intake （all P<0.01）, but the incidence rates of learning fatigue were lower than those with insufficient intake （all P<0.01）. The mental work ability index after class 8 of the students with adequate intake of phosphorus and sodium were higher than those with excessive intake （all P<0.01）, but the incidence rates of learning fatigue were lower than those with excessive intake （all P<0.01）. Conclusion Vocational college students have low intake of dietary nutrients such as nicotinic acid and vitamin C, but high intake of vitamin E, phosphorus and sodium, and the unbalanced intake of nutrients is related to the decrease of mental work ability and the increase of incidence rate of learning fatigue.

Objective To study the toxic effects of exposure to anthraquinone on acute lethal effect, life span, fecundity and activity of antioxidant enzymes in Caenorhabditis elegans （C. elegans）. Methods Nematodes at L4 stage were exposed to anthraquinone （with the concentration of 0, 8, 20, 50, 125 μmol/L respectively based on the solubility of anthraquinone and acute toxicity test） and 1% dimethylformamide （DMF）, the lethality, life span, brood size and activity of enzymes were measured. Results After 24 hours of acute exposure to anthraquinone, the nematode lethality of C.elegans increased with the increase of the dose, with the concentration of 20、50、125 μmol/L, the lethality rates were statistically different compared with the 1% DMF group（P<0.05 or P<0.01）. Meanwhile, the exposure of 8, 20, 50, 125 μmol/L, the increased activities of superoxide （SOD） were detected （P<0.01）. After long-term exposure to anthraquinone, compared with the 1% DMF group, exposure with the concentration of 125 μmol/L induced the reduction of brood size （P<0.05）. The average lifespan was decreased at the concentrations of 20, 50, and 125 μmol/L （P<0.05 or P<0.01）. Conclusion Exposure to anthraquinone can affect the lethality rate, lifespan and fecundity of C. elegans. In addition, anthraquinone can activate antioxidant enzymes and the oxidative defense system.

Objective To explore the correlation between serum adipocytokines and prognosis of cerebral infarction complicated with hypertension. Methods The patients with first-onset cerebral infarction complicated with hypertension treated in a hospital in Haikou from February 2018 to January 2021 were selected as the research object （cerebral infarction complicated with hypertension group）. At the same time, according to the same gender and age ± 3 years, the inpatients with cerebral infarction in the same hospital, the hypertensive patients in the hypertension clinic and the physical examination person in the healthy physical examination center were matched 1∶1 as the cerebral infarction group, the hypertension group and the healthy control group. Fasting venous blood was collected to detect the levels of visfatin, adiponectin （APN）, omentin-1 and leptin. The properties of carotid artery were measured by ultrasonic detector in the two groups other than the healthy control group and the hypertension group. The prognosis of patients with cerebral infarction complicated with hypertension was evaluated by modified Rankin Scale （MRS）. Descriptive epidemiological analysis method was used to analyze the relevant indexes of the four groups. Results A total of 248 patients with cerebral infarction complicated with hypertension were included in this study, including 148 males and 100 females, with an average age of （60.96 ± 4.77） years. There were 250 cases, 253 cases and 253 persons in cerebral infarction group, hypertension group and healthy control group respectively. There was no significant difference in gender distribution and age among the four groups （all P>0.05）. The levels of blood visfatin and leptin in the cerebral infarction complicated with hypertension group were higher, while the levels of omentin-1 and APN were lower than those in the cerebral infarction group, hypertension group, and healthy control group （all P<0.01）. The levels of blood visfatin and leptin in the cerebral infarction group were higher, while the levels of omentin-1 and APN were lower than those in the hypertension group and the healthy control group （all P<0.01）. The levels of blood visfatin and leptin were higher, while the levels of omentin-1 and APN were lower than those in the healthy control group （all P<0.01）. The levels of blood visfatin and leptin in the vulnerable plaque patients in cerebral infarction complicated with cerebral infarction group and hypertension group were higher, while the levels of omentin-1 and APN were lower than those in stable plaque patients （all P<0.01 ）. Six months after discharge, the levels of blood visfatin and leptin in patients with poor prognosis in cerebral infarction complicated with hypertension group were higher, and the levels of omentin-1 and APN were lower than those in patients with good prognosis （all P<0.01）. Serum omentin-1 and APN were negatively correlated with, while visfatin and leptin were positively correlated with the nature and prognosis of carotid plaque （all P<0.05）. Conclusion The level of serum adipocytokines in patients with cerebral infarction complicated with hypertension is closely related to the nature and prognosis of carotid plaque, which can provide a favorable basis for clinical follow-up diagnosis and treatment.

Objective To investigate the prevalence and influencing factors of autism spectrum disorders （ASDs） in preschool children aged 2-6 years. Methods Preschool children aged 2-6 years who participated in physical examination in a hospital in Wuhu City, Anhui Province from January to December 2019 were taken as the research object. The data were collected by questionnaire, the prevalence of ASDs was described, and the influencing factors of ASDs in preschool children in Wuhu were analyzed. Results A total of 13 482 children were surveyed, of which 12 657 children answered the questionnaire completely, 748 children with suspected ASDs were screened, and 52 cases were finally diagnosed. The prevalence rate of ASDs was 4.11‰. Multivariate Logistic regression analysis showed that boys （OR=1.422）, premature delivery （OR=1.582）, neonatal asphyxia （OR=1.416）, children's sleep disorders （OR=1.277）, mother’s childbearing age ≥35 years old （OR= 1.271）, mother's anxiety/depression during pregnancy （OR=1.439）, and the use of special drugs during pregnancy （OR=1.322） were risk factors for ASDs in preschool children. Conclusion Boys, premature delivery, neonatal asphyxia, children's sleep disorders, elderly maternal, negative emotions during pregnancy, and use of special drugs during pregnancy are the independent influencing factors of ASDs in preschool children. For some of the factors that can be artificially intervened, corresponding measures can be taken as soon as possible.

Objective To investigate the incidence of carotid artery stenosis （CAS） in patients with type 2 diabetes mellitus （T2DM） and to explore the related influencing factors for CAS. Methods The T2DM patients who first visited the endocrine clinic of a hospital in Xi'an from January 2018 to December 2020 were selected as the research object, data collection, physical examination, blood biochemical index detection and carotid ultrasound were carried out. Descriptive analysis was carried out on the detection of CAS in T2DM patients, and univariate and multivariate analysis was carried out on the influencing factors of CAS. Results Among 2 679 T2DM patients, 328 cases were complicated with CAS, and the detection rate was 12.24%. Multivariate Logistic regression analysis showed that older age （OR=1.039）, longer duration of diabetes （OR=1.115）, poor blood glucose control （OR=1.387）, dyslipidemia （OR=1.970） and hypertension （OR=3.725） were risk factors for CAS in T2DM patients. Conclusion T2DM patients with CAS are prominent. Attention should be paid to carotid ultrasound monitoring for T2DM patients with long duration of diabetes and poor blood glucose control. For diabetes patients with CAS risk factors, control and prevention methods should be carried out as soon as possible, so as to avoid the risk of cardiovascular and cerebrovascular diseases.

Objective To investigate the occupational stress and its influencing factors of operating room nurses in hospitals in Jinan City,so as to provide reference for controlling and alleviating the occupational stress of operating room nurses. Methods In March 2021,the occupational stress of operating room nurses in six hospitals in Jinan was investigated,the occupational stress scale（OSI-R）was used to obtain the occupational task score,individual stress response score and individual response ability score of operating room nurses. The demographic data of operating room nurses were collected,and the influencing factors of occupational stress of operating room nurses were analyzed. Results A total of 231 operating room nurses were included. In the OSI- R score,the occupational task score was（183.52±16.98）,the individual stress response score was（105.71±12.36）,and the individual response ability score was（121.53±11.94）.Multiple step-wise regression analysis showed that age （β ’= 1.963）,hospital level （β ’= -2.037）,position title（β’=1.942） and night shift frequency（β’=1.867）were the influencing factors of occupational task scores;education（β’=-1.925）, position title（β’=-2.476）,night shift frequency（β’=2.058）and chronic diseases（β’=2.119）were the influencing factors of individual stress response;age（β’=1.908）,education（β’= 2.017）,working years（β’=2.364）,physical exercise（β’=1.853）and chronic diseases（β’=-1.679）were the influencing factors of individual response ability. Conclusion The occupational stress of operating room nurses in Jinan City is at a high level,and the occupational task,indi- vidual stress response and individual response ability are affected by many factors. Intervention measures should be taken to alleviate the occupational stress of operating room nurses.

Objective To analyze the characteristics of new cases of lung cancer in the northeast negion of Sichuan from 2016 to 2020, so as to provide scientific basis for the prevention and treatment of lung cancer in this region. Methods The data of 3 146 new cases of lung cancer in the northeast negion of Sichuan were derived from two medical record management information systems in Nanchong from 2016 to 2020. The new cases of lung cancer were analyzed by descriptive epidemiological analysis method. Results Among the 3 146 new cases of lung cancer, there were 570, 604, 591, 658 and 723 cases in 2016, 2017, 2018, 2019 and 2020 respectively, and the number of cases increased year by year. Male cases accounted for 53.3%, mainly aged 60-76 years （47.5%）. There was no significant difference in gender and histopathological classification distribution of new lung cancer cases from 2016 to 2020 （all P>0.05）, but there was significant difference in age distribution （P<0.01）, and the incidence showed a younger trend. The main clinical manifestations were cough （75.08%）, hemoptysis or spit blood （31.98%）, chest pain （64.02%）, shortness of breath or wheezing （50.25%）, fever （39.00%） and dysphagia （20.79%）. Adenocarcinoma was the main histopathological classification （1 515 cases, accounting for 48.16%）, followed by squamous cell carcinoma （824 cases, accounting for 26.19%） and small cell carcinoma （560 cases, accounting for 17.80%）, and 247 other types, accounting for 7.85%. There was significant difference in histopathological classification of lung cancer patients between different genders from 2016 to 2020 （all P<0.01）. The histopathological classification of males was mainly squamous cell carcinoma and that of females was mainly adenocarcinoma. There were statistically significant differences in the distribution of histopathological classification of lung cancer cases of different ages in 2016, 2017 and 2018 （P<0.01 or P<0.05）. Conclusion The incidence of lung cancer in the northeast negion of Sichuan from 2016 to 2020 tends to be younger, with adenocarcinoma and squamous cell carcinoma more common, effective interventions should be taken according to the characteristics of lung cancer cases to reduce the disease burden.

Objective To evaluate the reliability and validity of SF-12v2 scale evaluating the quality of life of residents aged 40 and over in 6 counties and districts in Jiangsu Province, and obtain the reference data of standard model of residents aged 40 and over in Jiangsu Province. Methods Using a multi-stage stratified cluster sampling method, 3 583 SF-12v2 questionnaires were collected from 6 counties and districts in Jiangsu Province. Reliability was evaluated by Cronbach's α coefficient and Spearman-Brown split-half reliability coefficient, and validity was evaluated by factor analysis, the aggregation test and discrimination test. Results The Cronbach's α coefficient of SF-12v2 scale was 0.886 in 12 items, that of any dimension removed was between 0.810-0.841. The Cronbach's α coefficient of the physical component summary and the mental component summary was 0.767 and 0.759 respectively. Spearman-Brown split-half reliability coefficient was 0.795. The result of factor analysis showed that there were two principal components extracted by the method of the maximum variance orthogonal rotation, and the cumulative variance contribution rate was 61.2%. Among the eight dimensions of SF-12v2 scale, the lower limit of the aggregation test of each dimension was higher than the upper limit of the discrimination test, and the success rate of the aggregation test and discrimination test was both 100%. Conclusion The SF-12v2 scale was reliable and valid to evaluate the quality of life of residents aged 40 and over, and the scores of various dimensions can be regarded as the reference data of standard model for residents aged 40 and over in Jiangsu Province.

Objective To investigate the status of exclusive breastfeeding of 6-month-old infants in Hefei and analyze its influencing factors, so as to provide scientific basis for improving the behavior of exclusive breastfeeding. Methods Singleton live births delivered in the obstetrics department of a hospital in Hefei from January to October 2020 and their mothers were selected as the research object. At the age of 6 months after birth, the contact information of mother / father was obtained through hospital delivery records. The feeding status and influencing factors of 6-month-old infants were investigated. The feeding status of 6-month-old infants was analyzed by descriptive epidemiological analysis method, and the influencing factors of exclusive breastfeeding were analyzed by univariate and multivariate analysis methods. Results A total of 2 537 infants were included in the study. There were 724 cases of exclusive breastfeeding, accounting for 28.54%, 298 cases of dominant breastfeeding, accounting for 11.75%, 356 cases of supplementary feeding, accounting for 14.03%, 819 cases of breastfeeding, accounting for 32.28%, and 340 cases of bottle feeding, accounting for 13.40%.Multivariate Logistic regression analysis showed that the older the infant mother was （OR=0.441）, the depressed / flat nipple of the infant mother （OR=0.360）, and the milk opening time of the infant mother was ≥ 30 min （OR=0.431）, the possibility of exclusive breastfeeding of 6-month-old infants was less. The higher the educational background of the infant's mother （OR=2.499）, the occupation of public institution staff （OR=1.806）, the individual operator （OR=1.730）, the farmer / unemployed （OR=2.018）, and the infant's parents support breastfeeding （OR=2.392）, the possibility of exclusive breastfeeding of 6-month-old infants was higher. Conclusion The rate of exclusive breastfeeding of 6-month-old infants in Hefei is low, and it is affected by many factors such as mother's age, education, occupation, nipple depression/flat, milk opening time and whether father supports breastfeeding. According to the current situation and influencing factors, we should strengthen the education and intervention measures of exclusive breastfeeding to improve the situation of infant breastfeeding in this area.