高脂诱导妊娠期胰岛素抵抗大鼠模型的建立与评价

doi:10.12183/j.scjpm.2023.0824

华南预防医学 ›› 2023, Vol. 49 ›› Issue (7): 824-827.doi: 10.12183/j.scjpm.2023.0824

高脂诱导妊娠期胰岛素抵抗大鼠模型的建立与评价

滕瑶瑶¹, 陈荣², 金红梅¹, 梅珊珊³, 云雪霞⁴, 龙燕³, 黄耀刚¹, 陈逸升¹, 赵学芹³, 杨红玲³, 朱春燕¹

1.广州医科大学公共卫生学院,广东广州 511436;
2.广州医科大学附属第五医院;
3.广州市妇女儿童医疗中心;
4.广州市疾病预防控制中心

收稿日期:2022-09-06 出版日期:2023-07-20 发布日期:2023-10-12
通讯作者: 朱春燕,E-mail: zchyan@163.com
作者简介:滕瑶瑶（1997—）,女,在读硕士研究生,研究方向为公共卫生
基金资助:
广东省自然科学基金项目（2021A1515012200,2022A1515011550,2016A030313497）; 广东省高校科研项目（2021KTSCX094）; 广州医科大学大学生创新能力提升项目（2022）

Establishment and evaluation of a rat model of gestational insulin resistance induced by high-fat diet

TENG Yaoyao¹, CHEN Rong², JIN Hongmei¹, MEI Shanshan³, YUN Xuexia⁴, LONG Yan³, HUANG Yaogang¹, CHEN Yisheng¹, ZHAO Xueqin³, YANG Hongling³, ZHU Chunyan¹

1. School of Public Health, Guangzhou Medical University, Guangzhou 511436, China;
2. The Fifth Affiliated Hospital of Guangzhou Medical University;
3. Guangzhou Women and Children's Medical Center;
4. Guangzhou Center for Disease Control and Prevention

Received:2022-09-06 Online:2023-07-20 Published:2023-10-12

摘要/Abstract

摘要： 目的通过高脂喂养方法建立一种类似于人类妊娠糖尿病的妊娠期胰岛素抵抗大鼠模型。方法将12只SPF级雌性未经产SD大鼠随机分为2组,普通饮食组大鼠在妊娠前后喂养正常饮食,高脂饮食组大鼠在妊娠前后喂养高脂饮食。测量饮食干预前、孕前高脂饮食期及孕期的体重及血糖,并于妊娠第18天进行空腹葡萄糖耐量试验。结果妊娠第5、12及18天,高脂饮食组大鼠空腹血糖较普通饮食组大鼠高,差异均有统计学意义（P<0.05或P<0.01）。高脂饮食组大鼠口服葡萄糖耐量试验的所有时间点血糖均高于普通饮食组大鼠（P<0.05或P<0.01）。高脂饮食组大鼠妊娠第18天的空腹胰岛素、胰岛素抵抗指数、游离脂肪酸、低密度脂蛋白胆固醇及三酰甘油较普通饮食组大鼠高,差异均有统计学意义（P<0.05或P<0.01）。结论高脂喂养复制的妊娠期糖尿病SD大鼠模型,孕鼠从妊娠早期血糖升高且持续至妊娠晚期,同时存在明显的胰岛素抵抗,这些特征与人类妊娠期糖尿病的发病特征相似。

关键词: 妊娠期糖尿病, 高脂饮食, 胰岛素抵抗, 动物模型

Abstract: Objective To establish a rat model of gestational insulin resistance similar to human gestational diabetes mellitus by feeding a high-fat diet. Methods Twelve SPF-grade unproductive female SD rats were randomly divided into a normal diet group （NP group） and a high-fat diet group （HP group）. Before and after pregnancy, the NP group was fed with a normal diet, while the HP group with a high-fat diet. The body weight and blood glucose were measured before dietary intervention, during pregestational high-fat diet period, and during pregnancy. Fasting glucose tolerance test was performed on the 18^th day of pregnancy. Results On the 5^th, 12^th, and 18^th day of pregnancy, fasting blood glucose in HP group was significantly higher than that in NP group （P < 0.05 or P < 0.01）. The blood glucose in HP group was significantly higher than that in NP group at all time points of oral glucose tolerance test （P < 0.05 or P < 0.01）. The levels of insulin, insulin resistance index, free fatty acid, low density cholesterol, and triglyceride in HP group were significantly higher than those in NP group on the 18^th day of pregnancy （P < 0.05 or P < 0.01）. Conclusions In the SD rat model of gestational diabetes mellitus induced by a high-fat diet, the blood glucose of pregnant rats increased from early pregnancy and maintained highly until late pregnancy, and there was obvious insulin resistance. These characteristics were similar to the pathogenesis of human gestational diabetes mellitus.

Key words: Gestational diabetes mellitus, High-fat diet, Insulin resistance, Animal model

中图分类号:

R111

滕瑶瑶, 陈荣, 金红梅, 梅珊珊, 云雪霞, 龙燕, 黄耀刚, 陈逸升, 赵学芹, 杨红玲, 朱春燕. 高脂诱导妊娠期胰岛素抵抗大鼠模型的建立与评价[J]. 华南预防医学, 2023, 49(7): 824-827.

TENG Yaoyao, CHEN Rong, JIN Hongmei, MEI Shanshan, YUN Xuexia, LONG Yan, HUANG Yaogang, CHEN Yisheng, ZHAO Xueqin, YANG Hongling, ZHU Chunyan. Establishment and evaluation of a rat model of gestational insulin resistance induced by high-fat diet[J]. South China Journal of Preventive Medicine, 2023, 49(7): 824-827.

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高脂诱导妊娠期胰岛素抵抗大鼠模型的建立与评价

Establishment and evaluation of a rat model of gestational insulin resistance induced by high-fat diet

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