Objective To study the effects and mechanism of resveratrol(Res) on apoptosis and cell cycle in 3T3-L1 preadipocytes. Methods 3T3-L1 preadipocytes were treated with different doses of Restreatment (0,25,50,75,and 100μmol/L, 0μmol/Las the negative control) for 24, 48 or 72h. The Fluorescence microscope, MTT assay,and flow-cytometry were used for measuring cell morphology, cell proliferation, cell cycle, and apoptosis. Results After treated with 50μmol/L of Res for 48h, 3T3-L1 preadipocytes exhibited typical apoptosis features when observed under fluorescence microscope. MTT assay showed that, after different doses of Res treatment (0, 25, 50, 75, and 100μmol/L) for 24h, cell proliferation rate was 100%, (97.00 ±1.00)%, (91.00 ±2.65)%, (90.67 ±2.52)%, and (86.00±3.61)%, respectively; after the similar Res treatment for 48h, cell proliferation rate was 100%, (86.67±2.52)%, (76.00±2.00)%, (34.33±2.08)%, and(30.33±2.52)%, respectively; also, after the similar Res treatment for 72 h, cell proliferation rate was 100%, (82.00±2.65)%, (65.67±3.06)%, (21.00±3.61)%, and (16.33±3.21)%, respectively. Partial correlation analysis showed a negative correlation between cell proliferation rate and intervention time, concentration of Res (r=-0.72?-0.83, all P<0.01). After different doses of Res treatment (0, 25, 50, 75, and 100μmol/L) for 24h, the portion of cells in G0/G1 phase was (27.23±2.63)%, (39.03±2.74)%, (80.20±5.15)%, (87.97±3.12)%, (90.80±2.08)%, respectively; as the same, the portion of cells In S phase was (72.43±2.99)%, (63.93±6.90)%, (19.80±5.15)%, (12.20±2.86)%, (9.20±2.08)%, respectively. From the results of the cell cycle examination, the portion of cells in G0/G1 phase decreased (P<0.05orP <0.01) and increased evidently in S phase (P<0.05 or P < 0.01) in a dose dependent manner. After different doses of Res treatment (0, 25, 50, 75, 100μmol/L) for 48h, apoptosis rate was (2.90±0.10)%, (5.40±3.81)%, (8.23±4.24)%, (29.77±6.18)%, (27.23±3.17)%, respectively; as the same, necrosis rate was (7.50±0.87)%, (12.00±4.89)%, (12.27±3.81)%, (12.67±6.13)%, (20.73±2.64)%, respectively. The apoptosis and necrosis of 3T3-L1 preadipocytes were statistically significant different between 100μmol/L Res group and negative control group (P<0.05 or P<0.01). Conclusion At a certain dose range (0-100μmol/L), Res may inhibit the life cycle and promote apoptosis of 3T3-L1 preadipocytes.

Objective To build appropriate prediction model of influenza like illness (ILI) using Autoregressive Integrated Moving Average (ARIMA) modelMethods We collected the data of ILI surveillance from 2006 to 2011 in Nanshan District, Shenzhen, and built ARIMA model according to Akaike’s Information Criterion (AIC) and Bayesian Information Criterion (BIC). The autocorrelation analysis and Partial correlation analysis were used to identify the model. The model diagnosis was performed using Q statistic analysis.The actual ILI surveillance data in 2012 were compared with predictive value of the model to evaluate its predictive effect. Results A total of 199 360 ILI cases were reported from 2006 to 2011.The month max was 9765cases, the month min was 594 cases, and the average was 2769 cases per month. The annual incidence of ILI cases presented obvious peaks and valleys in 2006-2011. The incidence peak was from May to August and the incidence valley was from November to February each year. Relatively smooth sequence was obtained and suitable for model fitting. ARIMA (0,1,1)×(0,0,1)12 was selected as the optimal model.AIC and BIC values were the least, 1239.19 and 1245.98, respectively. The Q statistic was 19.07 (P>0.05) by Box-Ljung testing, indicating the applicability of the model. There was no statistically significant difference between the observed value in 2012 and predicted value (P>0.05). Conclusion ARIMA model is suitable for prediction of ILI incidence

Objective To analyze the dynamic blood lead levels in workers exposed to lead fume and dust in a large-scale enterprise manufacturing lead accumulators during the past 12 years, and evaluate the effect of prevention on occupational lead poisoning. Methods The on-site occupational health investigation was conducted to understand the manufacture process and basic occupational health situation. The lead fume (dust) concentration at workplace were detected by the flame atomic absorption spectrometry (FAAS) and the blood lead level of workers was detected by the graphite furnace atomic absorption spectrometry (GFAAS). The results were evaluated according to GBZ2.1-2007 and GBZ37-2002, respectively. Results The range of time-weighted average concentration of lead dust was 0.002-3.105 mg/m3 and its median was 0.047 mg/m3, the range of qualified rate was 45.0% -83.3%, and the average qualified rate was 61.7% (187/303). While the range of time-weighted average concentration of the lead fume was 0.002-2.523 mg/m3 and its median was 0.039mg/m3, the range of qualified rate was 20.0%-100%, and the average qualified rate was 49.0% (73/149) since the lead accumulator manufacturer put into production (2001-2012). The qualified rates of lead fume and dust were 31.8% -88.9% and the average qualified rate was 57.7% (260/452). The differences of qualified rates in different years were statistically significant (P<0.01). The qualified rate of lead fume (dust) rose from31.8% in 2002 to 88.9% in 2011. A total of 7893 workers had regular occupational health examination in the past 12 years. The range of blood lead concentration was 0.080-4.213 μmol/L and the average was (1.098±0.464)μmol/L. The blood lead content of men (1.131±0.440 μmol/L) was higher than that of women (0.727±0.340 μmol/L) (P<0.01). The mean blood lead levels of workers showed a significant downtrend with the growth of years (R=-0.755, P<0.01). The constituent ratios of different blood lead levels of <1.9, 1.9-2.9, and >2.9 μmol/L in workers were 94.83%, 4.78%, and0.39%, respectively. The concentrations of blood lead in workers were classified as < 1.9 μmol/L (normalrank) and ≥ 1.9 (higherrank) to conduct the Chi-squaretest. The differences between two ranks of blood lead levels in different years were statistically significant (P<0.01). The blood lead level of exposed workers at rank of < 1.9 μmol/L was up to 100.00% in 2012 from 80.99% in 2001. Seven workers were diagnosed with chronic occupational lead poisoning, including 5 mild and 2 moderate cases. Conclusion The concentration of lead fume (dust) and the blood lead level of exposed workers declined year by year since enterprise starting manufacturing the lead accumulators. The exceeding standard rate of blood lead and incidence rate of chronic occupational lead poisoning were low.The effect of prevention on chronic occupational lead poisoning was remarkable.

Objective To analyze the main causes of death and the potential years of life lost (PYLL) among residents in Guangzhou in 2011. Methods The population data in 2011 were provided by the Health Bureau of Guangzhou City. The data of death causes of Guangzhou in 2011 were obtained from the registry and report system of death causes. The causes of death were classified according to ICD-10. The leading causes of death and the potential life loss among residents in Guangzhou in 2011 were evaluated by the mortality rate, life expectancy, cause-deleted life expectancy and PYLL. Results There were 44262 cases of deaths in Guangzhou in 2011. The mortality rate was 544.47/100000 (standardized rate was 336.45/100000) in 2011.The mortality rates were 612.50/100000 (standardized rate was 431.12/100000) in male and 474.66/100000 (standardized rate was 248.26/100000) in female. The mortality in male was significantly higher than that in female (P<0.01). The six leading causes of death were malignant neoplasms, heart diseases, respiratory diseases, cerebrovascular diseases, acatalepsy, and injury, and their death rates were 133.44/100000, 101.82/100000, 100.68/100000, 73.70/100000, 31.54/100000, and 26.53/100000, respectively. The life expectancy was 80.83 years. It would increase 3.81, 2.78, and 2.73 years if removing the three leading causes of death. Their PYLL were 107716.00, 81414.00, 81106.50, 61978.50, 26523.00, and 22232.50 years. Conclusion The chronic diseases, especially, extraordinary malignant neoplasms, heart diseases, and respiratory diseases were the main causes of deaths in Guangzhou in 2011. The prevention and treatment of malignant neoplasms, heart diseases, respiratory diseases should be the focus of healthcare in Guangzhou. In particular, the prevention of tumorigenesis and the early diagnosis and treatment are crucial to the reduction of population life lost.

Objective To understand HIV positive rate and its correlation among voluntary counseling and testing (VCT) in Zhongshan City, Guangdong Province. Methods The VCT data during 2010 to 2012 in Zhongshan City were downloaded from China information system for disease control and prevention. HIV and syphilis serological detection were performed for all VCT participants. The HIV positive rates were analyzed using the single factor and multifactor unconditioned logistic regression. Results Three VCT sites received 3613 clients during 2010 to 2012. Of the 3601 (99.7%) clients having HIV test, 212 (5.9%) were positive. The HIV positive rates were 5.8% (173/2972) for male and 6.2% (39/629) for female. Of the 2759 clients having syphilis test, 121 (4.4 %) were positive. The syphilis seropositive rate was 4.9% (117/2 389) for male and 1.1% (4/370) for female. The HIV positive rate for syphilis seropositive clients was 25.6% (31/121). The HIV positive rates were 66.7% (8/12) for those suspected positive in history test, 17.6% (3/17) for those with blood transfusion or sale history, 13.1% (29/221) for those having a positive regular sexual partner, 12.7% (8/63) for those injecting drug use, 10.4% (114/1 096) for those men who had sex with men (MSM), 6.3% (3/48) for those with sex transmitted disease history, 5.9% (4/68) for those having a regular sexual partner with high risk behaviors, 3.0% (1/33) for those whose mothers were HIV positive, 2.5% (22/896) for those with unmarried heterosexual behaviors, and 2.4% (20/825) for those with commercial heterosexual behaviors. Clients with occupation exposure, positive family members except for the mother, unsafe behaviors such as exelcymosis and needling, health examination, or fear of AIDS were all screened to be HIV negative. The multifactor logistic regression model showed that, the lower level of education (AOR=1.39), had a higher risk to be HIV positive; clients with syphilis seropositive tests were 4.14 times the risk to be HIV positive compared to those with syphilis seronegative tests; MSM were 4.78 times the risk to be HIV positive compared to those with unmarried heterosexual behaviors, and those with a positive regular sexual partner were 4.71 times the risk to be HIV positive compared to those with unmarried heterosexual behaviors (all P<0.01). Conclusion Clients of the low level of education, the syphilis seropositive, MSM, and those with a positive regular sexual partner have a relative high HIV infection rate. It is necessary to take more comprehensive and effective prevention measures and intervention.

Objective To understand the influence factors of self health evaluation of residents in Zhuhai. Methods Stratified random sampling method was used to survey residents aged from 15 to 69 years old in 3 districts of Xiangzhou, Doumen and Jinwan of Zhuhai.Three common factors of social adaptability, physical status, and psychological status with 18 items of self health evaluation were surveyed by questionnaires. The general linear model was used for multivariate statistical analysis of influencing factors of self health evaluation. ResultsA total of 2 823 residents were surveyed, including 1385 men (49.1%) and 1438 women (50.9%). The average scores of social adaptability, physical status, psychological status, and total score of self health evaluation were 32.5±5.5, 24.4±4.2, 12.7±4.3, and 69.5±10.0, higher than their theoretical average ones (24, 18, 12, and 54, respectively). The factor of social adaptability was related to age（F=7.442）, medical condition（F=16.958）, family income（F=13.720）, and health insurance（F=6.982）(all P＜0.01）. The factor of physical status was related to sex（F=6.960） and medical condition（F=31.518）(all P＜0.01）. The factor of psychological status was related to occupation（F=7.017） and medical condition（F=74.950）(all P＜0.01）. Conclusion The influence factors of selfhealth evaluation of residents in Zhuhai included medical condition, family income, age, sex, and occupation.