Objective To investigate the levels of serum 25-hydroxyvitamin D [25-（OH）D] in children aged 0-6 years in Haikou and the relationship between vitamin D receptor （VDR） gene polymorphism and bone mineral density （BMD）. Methods Healthy children aged 0 - 6 years who underwent physical examination in Hainan Women and Children's Medical Center from January to December 2020 were selected as the subjects to detect the serum 25-（OH）D levels, VDR gene polymorphism and BMD. The serum 25-（OH）D levels of children with different gender, age, body mass index （BMI） and BMD, the VDR gene polymorphism of children with normal and abnormal BMD were compared, and the relationship between VDR gene polymorphism and BMD was analyzed. Results A total of 1 580 healthy children aged 0-6 years were investigated, including 838 boys and 742 girls, with an average age of （2.49±1.20） years. The serum 25-（OH）D level was （34.66±5.87） ng/mL, and the proportion of children with vitamin D deficiency, insufficiency, and sufficiency was 4.49%, 21.01%, and 74.49%, respectively. There was no significant difference in serum 25-（OH）D levels and vitamin D nutritional status between children of different genders （both P>0.05）. There were significant differences in serum 25-（OH）D levels and vitamin D nutritional status between children with different age groups, BMI and BMD （P<0.05 or P<0.01）. The results of genotyping showed that there were polymorphisms in APa Ⅰ locus of VDR gene in healthy children aged 0-6 years, and the genotypes were AA, Aa, and aa. There were significant differences in genotype and allele distribution of APaⅠ locus of VDR gene between normal and abnormal BMD children （all P < 0.01）. Multivariate Logistic regression analysis showed that children with Apa Ⅰ genotype aa of VDR gene （OR = 3.729） and a allele （OR = 2.656） had a higher risk of abnormal BMD. Conclusion The level of serum 25-（OH）D in children aged 0-6 years in Haikou is related to children's age, BMI and BMD, and the VDR gene polymorphism in children is closely related to the occurrence of abnormal BMD.

Objective To study the status and influencing factors of AIDS-related knowledge, attitude, and practice among college students in Xinyang City, so as to strengthen the education and publicity of AIDS among college students and enhance their self-protection ability. Methods In April 2021, a convenient sampling method was used to conduct a questionnaire survey on AIDS-related knowledge, attitude, and practice among college students in 3 colleges and universities in Xinyang City, and the results were analyzed by the descriptive analysis method. Univariate and multivariate analysis methods analyzed the influencing factors of AIDS-related knowledge, attitude, and practice scores. Results A total of 1 219 students were included in the survey. The average score for AIDS-related knowledge was （17.02±3.95）, for AIDS-related attitude was （86.71±9.20）, and for AIDS-related risky practice was （11.30±2.16）. Multiple linear regression analysis showed that grade （β'=2.014）, major （β'=-0.291）, origin （β'=0.109）, and history of sexual behavior （β'=0.765） were the influencing factors of AIDS-related knowledge scores among college students. Grade （β'=0.096）, major （β'=-0.180）, origin （β'=0.281）, history of sexual behavior （β'=0.369）, and AIDS-related knowledge score （β'=0.281） were the influencing factors of AIDS-related attitude scores among college students. Gender （β'=0.638）, grade （β'=0.142）, major （β'=-0.242）, origin （β'=-0.357）, history of sexual behavior （β'=-0.658）, history of urogenital diseases （β'=0.257）, AIDS-related knowledge score （β'=-0.276）, and AIDS-related attitude score （β'=-0.307） were the influencing factors of AIDS-related risky practice scores among college students. Conclusion The AIDS-related knowledge, attitude, and practice of college students in Xinyang City are affected by a variety of factors, and relevant health education measures should be strengthened to improve the level of AIDS-related knowledge and attitude of college students effectively and reduce the occurrence of risky practice, promote AIDS prevention and control.

Objective To investigate the compliance of cancer pain treatment in tumor patients and its influencing factors. Methods Patients who received cancer pain treatment in a Hospital of Chengdu from January to October 2019 were selected to investigate their compliance status, and the influencing factors were analyzed by univariate and multivariate analysis methods. Results Among the 195 patients, there were 122 males and 73 females, with an average age of （63.10±11.72） years. The score of compliance of cancer pain treatment was （0.91±1.40）, and there were 120 patients with good compliance （61.5%）. The results of binary logistic regression analysis showed that the settlement method at one's own expense （OR=0.486）, the longer the course of the tumor （OR=0.561）, and no complications （OR=2.395） were the influencing factors for the compliance of tumor patients with cancer pain treatment. Conclusion The compliance of cancer pain treatment in tumor patients needs to be improved. Medical staff should pay more attention to the influencing factors of compliance and take targeted measures to improve the compliance of patients.

Objective To analyze the current status and influencing factors of renal function decline among community elderly, so as to provide a reference for active prevention and treatment of renal function decline. Methods The convenience sampling method was used to select ten communities within the jurisdiction of Danzhou City, and each community was expected to survey 500 residents aged ≥60 years for questionnaire survey, physical examination, and detection of biochemical blood indicators and renal function-related indicators. The descriptive epidemiological analysis method was used to analyze the incidence of renal function decline among elderly, and univariate and multivariate analysis methods were used to analyze the influencing factors of renal function decline in this population. Results A total of 4 921 residents aged ≥60 years in Danzhou City were collected, including 2 569 males and 2 352 females, aged 60-87 years, with an average of （68.9±4.8） years old. Three hundred ninety cases of decreased renal function were detected, and the detection rate was 7.93%. Multivariate Logistic regression analysis showed that gender （OR=1.634）, age （OR=1.960）, body mass index≥24.0 kg/m² （OR=1.732）, drinking history （OR=1.128）, physical exercise （OR=0.369）, anemia （OR=1.701）, hypertension （OR=2.672）, diabetes （OR=3.758）, dyslipidemia （OR=3.174）, and abnormal liver function （OR=1.436） were the influencing factors of renal function decline among the elderly population in Danzhou City, Hainan Province. Conclusion The detection rate of renal function decline among people aged ≥60 years in Danzhou City, Hainan Province, is high, and it is affected by multiple factors such as gender, age, drinking history, frequency of physical exercise, anemia, hypertension, dyslipidemia, and abnormal liver function. We should pay attention to the high incidence of renal function decline and take targeted intervention measures to avoid the risk of renal function decline.

Objective To explore the situation of poor vision and vision-related health behaviors among primary school students in Zhengzhou City. Methods A multi-stage stratified random sampling method was used to sample pupils in grades 4-6 in Zhengzhou City to survey vision examination and vision-related health behaviors. The descriptive epidemiological analysis method was used to analyze the vision status and vision-related health behaviors. Univariate and multivariate analysis methods were used to analyze the influencing factors of poor vision among school-age children. Results A total of 2 415 pupils in grades 4-6 in Zhengzhou City were examined for vision and investigated by questionnaire, including 1 221 boys and 1 194 girls, aged 10-13 years. There were 1 815 cases of poor vision, accounting for 75.16%. The proportion of mild visual impairment was the highest, with 1 156 cases, accounting for 47.87%, followed by moderate visual impairment （488 cases, 20.21%） and severe visual impairment （171 cases, 7.08%）. Multivariate Logistic regression analysis showed that gender （OR=0.517）, living in the city （OR=5.888）, living in school （OR=2.210）, reading and writing posture （OR=2.632, 4.699）, attending after-school tutoring classes （OR=5.501）, daily TV watching time （OR=1.440, 1.881, 2.389）, daily mobile phone or computer time （OR=2.307, 2.704, 3.747）, daily homework time （OR=1.736, 1.887, 2.234）, daily outdoor exercise time （OR=0.770, 0.422, 0.394）, and parents did not limit electronic video time （OR=2.019） were influencing factors for the poor vision of pupils in grades 4-6. Conclusion The poor vision of pupils in grades 4-6 in Zhengzhou City is serious. Preventing and controlling the risk of poor vision in school-age children can be achieved by accurately identifying dangerous behaviors with poor vision and giving individually targeted corrections.

Objective To analyze the status of multiple disease coexistence in patients with hypertension to provide reference data for preventing and treating hypertension. Methods Hypertensive patients treated and contracted for management in the hypertension department of a hospital in Zhengzhou from March 2019 to December 2020 were selected as the research subjects. A self-made questionnaire was used to collect hypertension complicated with other chronic diseases, and a descriptive analysis of the survey results was carried out. Results A total of 3 942 patients with hypertension were included in this study, including 2 048 males and 1 894 females, aged 37-78 years, with an average of （53.9 ± 6.8） years. The course of hypertension ranged from 1 to 18 years, with an average of （6.2 ± 1.9） years. Classification of hypertension： 1 172 cases of high blood pressure, 1 843 cases of grade 1 hypertension, 832 cases of grade 2 hypertension, and 95 cases of grade 3 hypertension. There were 1 847 cases of multiple diseases, with an incidence of 46.85%. Among them, 1 316 cases coexisted with one disease, accounting for 71.25%, 464 cases coexisted with two diseases, accounting for 25.12%, and 67 cases coexisted with three or more diseases, accounting for 3.63%. There were significant differences in the prevalence of multiple diseases coexistence among patients with hypertension of different gender, ages, smoking, drinking, physical exercise, course of hypertension, and grade of hypertension （P<0.05 or P<0.01）. The prevalence of multiple diseases coexistence was higher in males, 70-78 years old, smoking, drinking, physical exercise did not meet the standard, 16-18 years of course of hypertension, and grade 3 hypertension. In the coexisting diseases of hypertension, the top 3 were dyslipidemia （587 cases, 31.78%）, type 2 diabetes （510 cases, 27.61%）, and coronary heart disease （432 cases, 23.39%）, followed by osteoporosis （384 cases, 20.79%）, chronic obstructive pulmonary disease （272 cases, 14.73%）, cerebral infarction （156 cases, 8.45%）, and chronic renal disease （113 cases, 6.12%）. Conclusion The proportion of hypertensive with multiple diseases is high, and there are many types of coexisting diseases. The common coexisting diseases are dyslipidemia, type 2 diabetes, coronary heart disease, and so on. Prevention and intervention should be strengthened according to the characteristics of hypertension patients with multiple diseases, active control of blood pressure while reducing the occurrence of coexisting diseases, so as to improve the quality of life of hypertensive patients.

Objective To analyze the cognitive status and its influencing factors of stroke prevention and control-related knowledge among community residents. Methods In October 2020, the convenient sampling method was used to select one district, county-level city, and county, respectively, in the jurisdiction of Tangshan, and one community （village） was selected from each district （county）. All adult permanent residents of the community （village） were investigated stroke-related knowledge. The descriptive analysis method was used to analyze the residents' mastery of stroke knowledge, and univariate and multivariate analysis methods were used to analyze the influencing factors. Results A total of 1 342 valid questionnaires were obtained from community residents in Tangshan City, including 685 males and 657 females, aged 18-84 years, with an average of （42.51±19.14） years old. The total score on the stroke prevention and control-related knowledge cognition scale was （27.69±5.13）, and the scoring rate of the total score was 38.89%. The score of basic knowledge of stroke was （18.25±4.25）, stroke-inducing factors was （4.86±1.00）, identifying risk factors of stroke was （4.77±0.96）, and knowledge of emergency treatment after stroke was （1.98±0.65）. The scoring rates from high to low were stroke-inducing factors, basic knowledge of stroke, identifying risk factors of stroke, and knowledge of emergency treatment after stroke, which was 45.45%, 42.86%, 38.46%, and 33.33%, respectively. Older age （β'=0.137）, higher education level （β'=0.119）, workers,cacles and professional technicians （β'=0.201）, stroke history/positive family history of stroke （β'=0.261、0.143）, positive history of related chronic diseases （β'=0.267）, and who have received stroke-related knowledge and health education （β'=0.328） had a higher level of knowledge about stroke prevention and control. Conclusion The cognition of stroke prevention and control-related knowledge of community residents in Tangshan needs to be improved. Actively improving community health education is an effective way to effectively improve the cognition level of stroke prevention and control-related knowledge of residents.

Objective To investigate breast carcinoma-related knowledge, attitude, and practice among hospital nurses in Zhengzhou City and analyze the influencing factors. Methods Taking the nurses from public medical institutions in Zhengzhou as the research object, in March 2021, the nurses from two tertiary hospitals and two secondary hospitals were selected by the convenience sampling method as the survey subjects to carry out a questionnaire on breast carcinoma-related knowledge, attitude, and practice. The descriptive analysis method was used to analyze the breast carcinoma-related knowledge, attitude, and practice of nurses, and univariate and multivariate analysis methods were used to analyze its influencing factors. Results The complete data of 311 nurses were collected. The lowest score for breast carcinoma-related knowledge was 9, and the highest score was 36, with an average of （21.69±5.47）. The lowest score for breast carcinoma-related attitude was 17, and the highest score was 42, with an average of （30.54±5.91）. The lowest score for breast carcinoma-related practice was 3, and the highest score was 14, with an average of （8.94±2.70）. Education background （β'=0.291）, professional title （β'=2.014 ）, department （β'=0.583）, nursing work experience （β'=0.226）, related training （β'=0.765 ）, family history of breast carcinoma （β'=0.180）, and history of breast disease （β'=0.657） were the influencing factors of breast carcinoma-related knowledge. Marital status（married β'=0.309）, department （β'=0.307）, nursing work experience （β'=0.462）, related training （β'=0.479）, family history of breast carcinoma （β'=0.448）, history of breast disease （β'=0.096）, and score of breast carcinoma-related knowledge （β'=1.973） were the influencing factors of breast carcinoma-related attitude. Age （β'=0.565）, marital status （married β'=0.309）, related training （β'=0.126）, family history of breast carcinoma （β'=0.357）, history of breast disease （ β'=0.159）, score of breast carcinoma-related knowledge （β'=2.106）, and score of breast carcinoma-related attitude （ β'=2.231） were the influencing factors of breast carcinoma -related practice. Conclusion The score of breast carcinoma-related knowledge, attitude, and practice of hospital nurses in Zhengzhou City is medium, which is affected by many factors. Targeted interventions should be taken for nurses in different situations to improve their breast carcinoma-related knowledge, attitude, and practice.

Objective To investigate the correlation and prevention significance of platelet （PLT）, glutaminyltransferase （GGT）, and interferon γ-inducible protein-10 （IP-10） levels with disease progression in patients with chronic hepatitis B virus infection. Methods Patients with chronic hepatitis B diagnosed and treated in a hospital in Shangqiu City from September 2019 to August 2021 were selected as the research subjects. According to different stages of disease progression, set up a simple chronic hepatitis B group （hepatitis group）, chronic hepatitis B post-cirrhosis group （liver cirrhosis group）, and chronic hepatitis B-related primary liver cancer group （liver cancer group）, and set up healthy people as the control group. Data collection and physical examination were performed on the research subjects. At the same time, fasting venous blood was collected for albumin （ALB）, globulin （GLB）, aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, PLT, GGT, IP-10, and other indicators detection. Pearson analysis method was used to analyze the correlation between PLT, GGT, IP-10, and liver function indicators, and the receiver operating characteristic curve （ROC） and area under ROC （AUC） were used to analyze the value of PLT, GGT, and IP-10 evaluating the liver cirrhosis and liver cancer. Results A total of 524 chronic hepatitis B patients with different disease progression stages were included, including 152 in the hepatitis group, 141 in the liver cirrhosis group, 131 in the liver cancer group, and 140 healthy people （control group）. There was no significant difference in age, gender, drinking history, smoking history, and past disease history among the four groups （all P>0.05）, but there was a significant difference in BMI distribution （P<0.01）. The test results and abnormal rate of AST, ALT, GGT, IP-10, ALB, GLB, and PLT between the four groups were statistically significant （all P<0.01）. PLT was negatively correlated with AST and ALT, and positively correlated with ALB and GLB （P<0.01）; GGT and IP-10 were positively correlated with AST and ALT, and negatively correlated with ALB and GLB （P<0.01）. The results of the ROC curve showed that the AUC of liver cirrhosis assessed by PLT and GGT combined with IP-10 was 0.894, which was more significant than PLT （0.828）, GGT （0.798）, and IP-10 （0.717）, and the AUC of liver cancer assessed by PLT and GGT combined with IP-10 was 0.945, which was more significant than PLT （0.854）, GGT （0.825）, and IP-10 （0.889）. Conclusion PLT, GGT, and IP-10 are dynamic changes in the process of chronic hepatitis B, liver cirrhosis, and liver cancer, which are closely related to the progress of chronic hepatitis B patients. They can be used as predictive markers of liver cirrhosis and liver cancer and provide a reference for monitoring and preventing chronic hepatitis B disease progression.

Objective To investigate the effects of PM_2.5 on oncogene expression via p38MAPK signaling pathway in human bronchial epithelial （HBE） cells. Methods Control group, PM_2.5 group, SB203580 group and PM_2.5+ SB203580 group were established. HBE cells were treated with 10 µmol/L p38MAPK inhibitor SB203580 for 30 min, then cultured in serum-free DMEM medium for 24 h as SB203580 group; HBE cells were treated with 50 μg/mL PM_2.5 for 24 h as PM_2.5 group; HBE cells were treated with10 µmol/L SB203580 for 30 min, then exposed to 50 μg/mL of PM_2.5 for 24 h as the PM_2.5+SB203580 group. The mRNA and protein expression levels of C-MYC, C-FOS, K-RAS, P53 and p38MAPK were detected by real-time PCR and Western blot. Results PCR results showed that compared with the control group, the mRNA expression of C-MYC, C-FOS, K-RAS, p38MAPK in the PM2.5 group increased by 54.0%, 49.2%, 96.1%, 74.1%, and P53 decreased by 49.1%. Compared with the PM_2.5 group, the mRNA expression of C-MYC, C-FOS, K-RAS and p38MAPK in the PM_2.5+SB203580 group decreased by 21.4%, 20.8%, 39.3%, 21.4%, and P53 increased by 43.1% （P<0.01 or P<0.05）. Western blot results showed that compared with the control group, the protein expression levels of C-MYC, C-FOS, K-RAS, p38MAPK in the PM_2.5 group increased by 110.6%, 46.7%, 35.5%, 82.8%, and P53 decreased by 42.7% （all P<0.01）. Compared with the PM_2.5 group, the protein expression levels of C-MYC, C-FOS, K-RAS, p38MAPK in the PM2.5+SB203580 group decreased by 37.7%, 16.2%, 19.6%, 25.9%, and P53 increased by 43.5% （P<0.01 or P<0.05）. Conclusion The p38MAPK inhibitor obviously influenced the effects of PM_2.5 on oncogene expression, indicating that p38MAPK signaling pathway plays an important role in PM_2.5-induced oncogene expression.

Objective To investigate the detection of myopia among children and adolescents in Chengdu and to explore the characteristics of eye-using behavior. Methods From September to October 2020, a multi-stage stratified cluster random sampling method was used to select primary and middle school students in Chengdu for a questionnaire survey and visual inspection. The descriptive analysis method was used to analyze the detection of myopia and eye-using behavior, and univariate and multivariate analysis methods were used to analyze the influencing factors of myopia. Results A total of 3 150 primary and middle school students were surveyed in this study, including 1 644 boys （52.19%） and 1 506 girls （47.81%）. The proportion of primary school students was the highest, accounting for 55.62%, and local registered residents accounted for 77.37%. Among them, 1 746 cases of myopia were detected, and the detection rate was 55.43%. Multivariate Logistic regression analysis showed that children and adolescents who often / always read and write with incorrect posture （OR=1.254）, use computers at close range （OR=2.998）, watch TV at close range （OR=2.225）, spend less than 2 hours outdoors every day （OR=2.886）, use eyes in bad environment or posture （OR=3.554）, and use electronic products for more than 2 hours every day （OR=3.102）, were more likely to develop myopia. Conclusion The detection rate of myopia among children and adolescents in Chengdu is relatively high, and the prevention of myopia among children and adolescents in Chengdu still needs to be strengthened. While encouraging them to increase outdoor activities and reduce electronic screens, they should correct incorrect reading and writing postures, intervene with eye-using behavior in close quarters and adverse environments, and form a healthy eye-using behavior habit.

Objective To explore the status and influencing factors of high-risk behavior among schizophrenic patients in Zhumadian. Methods The primary data of schizophrenic patients under the management of the psychiatry department of a hospital in Zhumadian City were collected as the research objects, and psychiatrists evaluated the high-risk behaviors of the survey subjects. The descriptive analysis method was used to analyze high-risk behavior in schizophrenic patients, and univariate and multivariate analysis methods were used to analyze the influencing factors of high-risk behavior. Results In this survey, 1 084 schizophrenic patients were evaluated for high-risk behavior, 679 were male, accounting for 62.64%, and 405 were female, accounting for 37.36%. The mean age was （47.89±27.65） years old. The evaluation results showed that 78 patients had high-risk behaviors, accounting for 7.19%. Schizophrenic patients who were male （OR=2.704）, aged 18-50 years old （OR=1.555, 2.729, 3.861）, nonlocal census register （OR=1.967）, unemployed or out of work （OR=2.729）, the guardian was not a spouse （OR=3.762, 4.250, 7.822, 8.306, 10.064）, more frequent hospitalizations in the past （OR=3.500, 3.803, 7.621）, and more frequent emergency treatment in the past （OR=10.644, 31.218） were more likely to have high-risk behaviors. Conclusion Schizophrenic patients in Zhumadian have a certain proportion of high-risk behaviors. The male, aged 18-50 years old, and nonlocal census register should actively implement assistance to ensure corresponding social support. For patients hospitalized many times and have had emergency treatment many times, we need to pay more attention.