Abstract: Objective To compare the life behavior and habits and gene polymorphism between young and middle-aged patients with ischemic stroke and healthy people. Methods A total of 617 young and middle-aged patients with ischemic stroke admitted to a hospital from January to December 2021 were selected as the case group, and 617 healthy people who underwent physical examination in the same hospital were selected as the healthy control group according to the ratio of gender and age of 1∶1. The differences in life behavior and habits and gene polymorphism between young and middle-aged patients with ischemic stroke and healthy people were compared. The life behavior and habits included weight control, smoking, drinking, sleep duration, physical exercise, and dietary health. The gene polymorphism was detected by polymerase chain reaction （PCR） and molecular hybridization for fibrinogen （FGB） G （-455） A, endothelial cell-leukocyte adhesion molecule （ELAM） ser128arg, cell adhesion molecule-1 （ICAM1） gly214arg, tumor necrosis factor α （TNFα） G （-308） A, and tumor necrosis factor β （TNFβ） thr26asn. Results In the case group, there were 396 males and 221 females, the age was 37-64 years old. In the healthy control group, there were 396 males and 221 females, the age was 37-64 years old. There were no significant differences in gender distribution, mean age, diastolic blood pressure, and systolic blood pressure between the two groups （all P > 0.05）. There were statistically significant differences in weight control, smoking, drinking, sleep duration, physical exercise, and dietary health between the two groups （P＜0.05 or P＜0.01）. There was no significant difference in the allele frequencies of FGB G （-455） A, ELAM ser128arg, ICAM1 gly214arg, TNFαG （-308） A, and TNFβ thr26asn between the two groups （ all P > 0.05）. There was no significant difference in the genotype frequencies of FGB G （-455） A, ELAM ser128arg, ICAM1 gly214arg, and TNFαG （-308） A between the two groups （all P＞0.05）, while the difference in the genotype frequency of TNFβ thr26asn was statistically significant （P＜0.05）. Conclusions Young and middle-aged ischemic stroke have genetic factors, which may be related to TNFβ thr26asn polymorphism. It is beneficial to the prevention and control of ischemic stroke in young and middle-aged people to seek genetic etiology evidence and establish good living habits, such as weight control, quitting smoking and alcohol restriction, ensuring adequate sleep, adhering to physical exercise, and a healthy diet.

Key words: Ischemic stroke, Young and middle-aged, Healthy people, Life behavior and habits, Gene polymorphism