ObjectiveTo understand knowledge about HIV/AIDS and sexual behavior among men who have sex with men(MSM) in Miangyang City, and to analyze factors influencing HIV infection.MethodsFrom February to June in 2017, MSM were recruited by using snowball sampling method in Mianyang City, Sichuan Province to conduct anonymous questionnaire survey.Univariate and multivariate logistic regressions were used to analyze factors influencing HIV infection. ResultsA total of 400 MSM were investigated. The total awareness rate of HIV/AIDS knowledge was 97.0%, the coverage rate of intervention service was 88.8%, the rate of anal sex among MSM was 87.3% in recent 6 months, the rate of commercial sexual behavior was 2.0%, and the rate of heterosexual behavior was 14.5%. Of all the MSM, 89.6%(355/396) sought sexual partners by using the internet/dating software. The confirmed positive rate of HIV infection was 6.8%. Multivariate logistic regression showed that those with college or above education（OR=0.156）and those who received condom promotion and distribution/HIV counseling and testing in recent year（OR=0.286）had lower risk of HIV infection,while those with uncertain sexual orientation（OR=16.107）and those with unprotected anal sex behavior in recent 6 months（OR=2.832）had higher risk of HIV infection.ConclusionThe awareness rate of AIDS/HIV was high in Mianyang City in 2017. Those with low levels of education, uncertain sexual orientation, and unprotected anal sex had greater risk of HIV infection.

ObjectiveTo evaluate feeding indexes of infants aged 0 to 36 months in Guangdong Province and assess feeding behaviors.MethodsMultistage random sampling method was used to select children aged 0-36 months from a metropolis，middle-sized city, and rural area of Guangdong Province．Basic information and food consumption of the subjects were investigated by questionnaire survey. Based on the standard for infant and child feeding index(ICFI) score recommended by China Center for Disease Control and Prevention, ICFI scores of children aged 0 to 36 months in Guangdong Province were evaluated.ResultsA total of 1 447 infants and young children were investigated, including 761 males(52.6%) and 686 females(47.4%); 64.3%(930/1 447) were aged 12-36 months. Breastfeeding rates of children aged 0-5, 6-8, 9-11, and 12-36 months were 84.8%(224/264), 58.2%(82/141), 34.9%(38/109), and 6.6%(57/859), respectively. With the increase of age, the scores of beans, eggs, fish, meat and food diversity increased. The total qualified rate of ICFI was 75.0%(1 030/1 373）. The ICFI scores of children aged 0-5, 6-8, 9-11, and 12-36 months were(1.70±0.72),(6.07±2.29),(7.38±1.81), and(8.94±1.63), and the qualified rates of ICFI were 84.8%, 28.4%, 49.5%, and 82.9%, respectively. The differences in the ICFI and qualified rates of ICFI were statistically significant between different age groups( both P＜0.01). The ICFI scores and qualified rates of ICFI in metropolis and middle-sized city were significantly higher than those in rural area( both P＜0.01).ConclusionThe feeding behavior of infants and children aged 0-36 months in Guangdong Province needs to be improved. Mothers of infants and young children in rural areas and aged 6-8, 9-11 months should increase the variety of supplementary foods.

ObjectiveTo understand the status and influencing factors of dietary behaviors among teenagers at school in Zhongshan City.MethodsStudents were selected from six full-time universities and middle schools in Zhongshan City by stratified cluster random sampling to conduct a survey of Questionnaire on Health related behaviors of adolescents in China.ResultsA total of 2 341 students were investigated, including 1 152 boys and 1 189 girls; 594 junior middle school students, 530 high school students, 636 vocational middle school students, and 581 university students. The percentages of unhealthy dietary behaviors of drinking beverage every day, eating dessert every day, eating fried food every day, often eating fast food, enjoying food of street vendors frequently, and dietary bias were 12.7%, 14.3%, 5.3%, 6.9%, 12.5%, 34.4%, respectively. The rates of healthy dietary behaviors of eating fruits every day, eating vegetables every day, having breakfast regularly, and drinking milk frequently were 41.1%, 73.6%, 81.2%, and 35.2%, respectively. The rates of drinking soft beverages and eating western-style fast food in male students were higher than those in female students(P<0.01 or P<0.05), while the rates of eating dessert, eating fruits every day, and having breakfast in female students were higher than those in male students(all P<0.01). Compared with boarding students, commuter students had higher rates of drinking soft beverages every day, eating fried food every day, eating western-style fast food, eating fruit and vegetables every day(P < 0.01 or P < 0.05). On the contrary, compared with the commuter students, the boarding students tended to drink milk frequently and having dietary bias(both P < 0.01). The higher the education level of the mothers, the higher the rates of healthily eating behaviors of the students who took vegetables, fruits and milk frequently(all P<0.01).ConclusionUnhealthy dietary behaviors were prevailing among teenagers at school in Zhongshan. Relevant interventions should be taken in accordance with their features to deepen teenagers' understanding of healthy dietary behavior and promote them to build healthy dietary habit.

ObjectiveTo explore the spatiotemporal distribution characteristics and trends of centenarians in Hechi.MethodsSpatial database of centenarians in Hechi City from 1982 to 2010 were established and analyzed by using the geographic information system software Arcgis 10.4 for Kriging interpolation analysis and trend surface analysis.ResultsThe proportions of centenarian in four censuses (1982-2010) in Hechi City were 3.59/10.5, 6.89/10.5, 8.43/10.5 and 12.50/10.5, respectively. The percentage of female centenarians was higher than that of male ones. The centenarians were mainly concentrated in three ethnic groups: Zhuang, Yao and Han. Among them, the number of centenarians in Zhuang and Yao accounted for 83.55% of the total centenarians. Centenarian ratio in Yao always maintained between 19.05/10.5 and 22.41/10.5. Most of the centenarians were illiterate. The results of Kriging interpolation showed that in the past thirty years, the longevity level in the southwest of Hechi(Bama, Fengshan and Donglan) was always higher than that in other areas. The CH value of the southwest of Hechi was increasing(up to 35.61/10.5) and the scope of areas with higher CH value was expanding. The trend surface analysis showed that the level of longevity in Hechi City gradually increased from east to west and weakened from south to north, though the trend in the north-south direction in 2010 showed a weak inverted "U" type.ConclusionAn obvious regional difference in longevity was observed in Hechi, and there existed a relatively stable longevity gathering area in the southwest of it. Factor analysis about longevity should be conducted based on the characteristics of spatial distribution and develop relevant health policies.

ObjectiveTo develop an evaluation index system for projects of essential public health service in Guangdong Province so as to provide an effective tool for performance evaluation.MethodsAn index system was preliminarily established using the literature analysis, and the relative weights of the first, second and tertiary indexes were calculated by using the percentile method and the product method through two rounds of expert letters.ResultsTwenty-five experts conducted the first round of expert consultation survey. Of all the experts, 17(68.0%) were engaged in the management of public health, 22(88.0%) had bachelor degrees or above, and 16(64.0%) had senior or associate-senior professional titles. There were 22 experts in the second round of expert consultation. Of them, 17(77.3%) experts were engaged in the management of public health, 20(90.9%) had bachelor degrees or above, 15(68.1%) had senior or associate-senior professional titles. The positive coefficients of two rounds of consulting experts were 83.3% and 88.0%, respectively. The average score of expert judgment was 0.813, the average degree of familiarity was 0.805, and the degree of the expert authority was 0.781. After two rounds of consultations, the performance evaluation index system of essential public health service projects in Guangdong Province was established, including the organization and management, fund management, project implementation, project effect, public awareness and satisfaction with 5 primary indexes, 25 secondary indexes, and 41 tertiary indexes.ConclusionThe evaluation index system was established in the study with high degrees of coordination and authority of the expert opinions, and can be used to evaluate the performance of essential public health service projects in Guangdong Province.

ObjectiveTo study the etiological analysis and molecular typing and traceability of Vibrio Parahaemolyticus isolated from a food poisoning event.MethodsAccording to "National food safety standard for food microbiological test: Vibrio Parahaemolyticus ” GB 4789.7-2013, specimens from the patients' stool, anal swabs and suspicious food in a food poisoning event were tested for Vibrio Parahaemolyticus. The strains isolated were tested for ToxR by real-time fluorescence PCR, and then the virulence gene of thermolabile hemolysin(tlh), thermostable direct hemolysin(tlh) and thermostable related hemolysin(trh) were tested by using multiplex fluorescent PCR. Electrophoresis fingerprints were obtained by pulsed field gel electrophoresis(PFGE) technique and the clustering analysis was performed by BioNumerics software.ResultsSix strains of Vibrio Parahaemolyticus were isolated from 4 patients' stool/anal swabs and 2 suspicious food. The serotypes were O1:KUT, O1:KUT, O3K6, O4:KUT, OUT:KIII, and O3:KUT, respectively. The specific ToxR gene was amplified from 6 strains of Vibrio Parahaemolyticus, all of which were positive for tlh gene. The 4 strains isolated from patient's stool/anal swabs were positive for the tdh virulence genes. One of 2 strains from the suspicious food was positive for tdh virulence gene. All strains were negative for trh virulence gene. Cluster analysis showed that the similarity of the strains was from 62.3% to 100.0%. The finger prints of strains isolated from suspicious food were different from those of strains from the specimens of the patients. Hence, they were different strains. The strains from patients with the same serotype were homologous in cloning. The strains belonging to different serotypes were different clones.ConclusionThe food poisoning event was caused by the mixed infection of different clones of Vibrio Parahaemolyticus, carrying the tdh virulence genes, with serotypes of O1, O3, and O4.