Abstract: Objective To analyze the HIV/AIDS sentinel surveillance data among college students in Guangdong Province from 2016 to 2021, with the aim of comprehending the prevalence of HIV and syphilis infections as well as discerning the evolving patterns of sexual behavior characteristics exhibited by college students in Guangdong Province, and to provide the reference for developing AIDS prevention and control measures in Guangdong Province. Methods A continuous cross‐sectional survey was conducted in cities with a high concentration of universities from 2016 to 2021. A phased cluster sampling method was used to collect anonymous questionnaires and blood samples from college students in selected areas, and to collect social demographics, sexual behavior information, and serological results of the survey subjects. The trend was analyzed using the Mantel‐Haenszel linear trend test. Results The detection rates of HIV/AIDS and syphilis among college students from 2016 to 2021 were 0 to 0.05% and 0 to 0.16%, respectively. The awareness rates of the severity of the disease and the main transmission routes of young students were both<80% （except in 2019）. The proportion of ever having sex, the proportion of having sex with permanent partners, temporary partners, commercial partners, homosexual partners in the last one year, the proportion of receiving intervention services in the last one year, the proportion of using condoms for the first sexual behavior, and the proportion of persisting condom use with permanent partners and temporary partners in the last one year showed a weak upward trend （r_s >0, P<0.05）. The average age of first sexual behavior was （20.00±1.89）. The first sexual behavior partners were mainly boyfriend/girlfriend （86.73%-92.91%）; the AIDS knowledge awareness rate of college students who had received intervention services in the last one year was higher than that of college students who had not received intervention services （P<0.01）. Conclusions From 2016 to 2021, the positive rates of HIV and syphilis antibodies among college students in Guangdong Province remained stable at a low level, and the characteristics and patterns of major sexual behaviors of college students did not change significantly. The HIV/AIDS propaganda and intervention work for college students has played a more obvious role, and the intervention for college students should be advanced.

Key words: HIV/AIDS, Syphilis, College student, Infection, Sexual behavior